Overview

  • Product nameAnti-Xrn1 antibody
    See all Xrn1 primary antibodies
  • Description
    Rabbit polyclonal to Xrn1
  • Tested applicationsSuitable for: ICC/IF, WB, IP, EMSAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    A region between residue 1650 and the C-terminus (residue 1706) of human Xrn1 (SwissProt Q6MZS8)

  • Positive control
    • DU145 cell line

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/5000 - 1/15000. Detects a band of approximately 194 kDa (predicted molecular weight: 194 kDa).
IP Use at 2-5 µg/mg of lysate.
EMSA Use at an assay dependent dilution. PubMed: 20691152

Target

  • RelevanceXrn1 is the major 5'-3' exoribonuclease involved in mRNA decay. Required for the 5'-3'processing of the G4 tetraplex-containing DNA and RNA substrates. The kinetic of hydrolysis is faster for G4 RNA tetraplex than for G4 DNA tetraplex and monomeric RNA tetraplex. Binds to RNA and DNA (By similarity). May act as a tumor suppressor protein in osteogenic sarcoma (OGS). Associates with alpha and beta tubulins (By similarity). Found in a mRNP complex with RENT1, RENT2, RENT3B and XRN1.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • 5'-3' exoribonuclease 1 antibody
    • Dhm2 antibody
    • mXRN1 antibody
    • SEP1 antibody
    • Strand-exchange protein 1 homolog antibody
    see all

Anti-Xrn1 antibody images

  • All lanes : Anti-Xrn1 antibody (ab70259) at 0.2 µg/ml

    Lane 1 : NIH3T3 whole cell lysate
    Lane 2 : TCMK-1 whole cell lysate
    Lane 3 : 4T1 whole cell lysate
    Lane 4 : CT26.WT whole cell lysate

    Lysates/proteins at 50 µg per lane.


    Predicted band size : 194 kDa


    Exposure time : 3 minutes

    Detection by chemiluminescence.

  • Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for immunoprecipitation, 20% of IP loaded) and 293T (T; 50 µg) cells. ab70259 used for western blot at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate.  

     
    XRN1 was also immunoprecipitated by ab70259. Detection was by chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

     

  • ICC/IF image of ab70259 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70259, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Xrn1 antibody (ab70259)

This product has been referenced in:
  • Moutaoufik MT  et al. UVC-induced stress granules in mammalian cells. PLoS One 9:e112742 (2014). Read more (PubMed: 25409157) »
  • Kim CW  et al. Tristetraprolin controls the stability of cIAP2 mRNA through binding to the 3'UTR of cIAP2 mRNA. Biochem Biophys Res Commun 400:46-52 (2010). EMSA ; Human . Read more (PubMed: 20691152) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

Submitted Dec 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"