The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: 1/2000 - 1/10000. Detects bands of approximately 109 and 117 kDa (predicted molecular weight: 109 kDa).
Is unsuitable for IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceXRN2 possesses 5'->3' exoribonuclease activity. XRN2 shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The function of the human gene has not yet been determined, however, it may promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.
All lanes : Anti-XRN2 antibody (ab72307) at 0.04 µg/ml
Lane 1 : Whole cell
lysate from HeLa cells at 50 µg Lane 2 : Whole cell
lysate from HeLa cells at 15 µg Lane 3 : Whole cell
lysate from HeLa cells at 5 µg Lane 4 : Whole cell
lysate from 293T cells at 50 µg Lane 5 : Whole cell
lysate from NIH 3T3 cells at 50 µg