Overview

  • Product nameAnti-XYLT2 antibody
    See all XYLT2 primary antibodies
  • Description
    Rabbit polyclonal to XYLT2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region of human XYLT2 (NP_071450).

  • Positive control
    • Fetal muscle lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

Target

  • FunctionProbably catalyzes the first step in biosynthesis of glycosaminoglycan. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes (By similarity). Its enzyme activity has not been demonstrated.
  • Tissue specificityWidely expressed. Expressed at higher level in kidney and pancreas.
  • PathwayGlycan metabolism; chondroitin sulfate biosynthesis.
    Glycan metabolism; heparan sulfate biosynthesis.
  • Sequence similaritiesBelongs to the glycosyltransferase 14 family. XylT subfamily.
  • Post-translational
    modifications
    Contains disulfide bonds.
  • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Peptide O xylosyltransferase 1 antibody
    • Peptide O-xylosyltransferase 1 antibody
    • protein xylosyltransferase 2 antibody
    • PXYLT2 antibody
    • UDP-D-XYLOSE:PROTEOGLYCAN CORE PROTEIN BETA-D-XYLOSYLTRANSFERASE 2 antibody
    • UDP-D-xylose:proteoglycan core protein beta-D-xylosyltransferase antibody
    • XT II antibody
    • XT-II antibody
    • XT2 antibody
    • Xylosyltransferase 2 antibody
    • Xylosyltransferase II antibody
    • xylT II antibody
    • XylT-II antibody
    • XYLT2 antibody
    • XYLT2_HUMAN antibody
    see all

Anti-XYLT2 antibody images

  • Anti-XYLT2 antibody (ab81311) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal Muscle Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 97 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References for Anti-XYLT2 antibody (ab81311)

ab81311 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Dog Cell lysate - whole cell (Kidney, epithelial)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 30 µg
Treatment Non treated, High glucose (30mM), high glucose + metformin (1mM) for 24 hrs
Specification Kidney, epithelial
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. srikanth

Verified customer

Submitted Mar 16 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"