Overview

  • Product name
    Y-27632 dihydrochloride
  • Description
    Selective Rho kinase inhibitor
  • Biological description
    Also available in simple stock solutions (ab144494) - add 1 ml of water to get an exact, ready-to-use concentration.
  • Purity
    > 99%

Properties

Applications

Our Abpromise guarantee covers the use of ab120129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab46154 staining VEGF in HeLa cells treated with Y-27632 dihydrochloride (ab120129), by ICC/IF. Decrease in VEGF expression correlates with increased concentration of Y-27632 dihydrochloride, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120129 (Y-27632 dihydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab46154 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

ab120129 has not yet been referenced specifically in any publications.

Customer reviews and Q&As



Y-27632 dihydrochloride, ab120129, is cell-permeable.

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We haven't tested our biochemical products for biological activity in-house and haven't filtered Y-27632, however the la...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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