Recombinant
RabMAb

Anti-Y14 antibody [EPR13945(B)] (ab181038)

Overview

  • Product name
    Anti-Y14 antibody [EPR13945(B)]
    See all Y14 primary antibodies
  • Description
    Rabbit monoclonal [EPR13945(B)] to Y14
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Y14 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y5S9

  • Positive control
    • HeLa, Jurkat, HepG2 and 293T cell lysates. Permeabilized Jurkat cells. Immunoprecipitation pellet from Jurkat cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
IHC-P 1/250 - 1/500.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/10 - 1/100.

Target

  • Function
    Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Remains associated with mRNAs in the cytoplasm until the mRNAs engage the translation machinery. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. Complex with MAGOH is a component of the nonsense mediated decay (NMD) pathway.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the RBM8A family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization
    Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Binder of OVCA1 1 antibody
    • Binder of OVCA1-1 antibody
    • BOV 1 antibody
    • BOV 1A antibody
    • BOV 1B antibody
    • BOV 1C antibody
    • BOV-1 antibody
    • BOV1 antibody
    • BOV1A antibody
    • BOV1B antibody
    • BOV1C antibody
    • HSPC 114 antibody
    • HSPC114 antibody
    • MDS 014 antibody
    • MDS014 antibody
    • RBM 8 antibody
    • RBM 8A antibody
    • RBM 8B antibody
    • RBM8 antibody
    • rbm8a antibody
    • RBM8A_HUMAN antibody
    • RBM8B antibody
    • Ribonucleoprotein RBM 8 antibody
    • Ribonucleoprotein RBM 8A antibody
    • Ribonucleoprotein RBM8 antibody
    • Ribonucleoprotein RBM8A antibody
    • RNA binding motif protein 8 antibody
    • RNA binding motif protein 8A antibody
    • RNA binding motif protein 8B antibody
    • RNA binding protein 8A antibody
    • RNA binding protein Y14 antibody
    • RNA-binding motif protein 8A antibody
    • RNA-binding protein 8A antibody
    • RNA-binding protein Y14 antibody
    • ZNRP antibody
    • ZRNP 1 antibody
    • ZRNP1 antibody
    see all

Images

  • All lanes : Anti-Y14 antibody [EPR13945(B)] (ab181038) at 1/1000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : Jurkat cell lysates
    Lane 3 : HepG2 cell lysates
    Lane 4 : 293T cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 19 kDa

  • Flow cytometric analysis of permeabilized Jurkat cells using ab181038 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Western blot analysis on immunoprecipitation pellet from (Lane 1) Jurkat cell lysate or (Lane 2) 1XPBS (negative control) using ab181038 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

References

This product has been referenced in:
  • Shadle SC  et al. DUX4-induced dsRNA and MYC mRNA stabilization activate apoptotic pathways in human cell models of facioscapulohumeral dystrophy. PLoS Genet 13:e1006658 (2017). Read more (PubMed: 28273136) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (fibroblast)
Permeabilization
Yes - 0.3% Triton X-100 in blocking buffer
Specification
fibroblast
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 12 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (Lymphocytes)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
40 µg
Specification
Lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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