The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 65 kDa).Can be blocked with Human YAP1 peptide (ab39360).
1% milk blocking recommeded
Use a concentration of 1 µg/ml.
FunctionTranscriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).
Tissue specificityIncreased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
Sequence similaritiesBelongs to the YORKIE family. Contains 2 WW domains.
Post-translational modificationsPhosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage.
Cellular localizationCytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density.
ICC/IF image of ab39361 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab39361 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
IHC image of YAP1 staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39361, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-YAP1 antibody (ab39361)
All lanes : Anti-YAP1 antibody (ab39361) at 1 µg/ml
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate with 5% BSA blocking Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate with 5% BSA blocking Lane 3 : LS147T (Human colon cancer) Whole Cell Lysate with 5% BSA blocking Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate with 1% milk blocking Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate with 1% milk blocking Lane 6 : LS147T (Human colon cancer) Whole Cell Lysate with 1% milk blocking
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)) developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 65 kDa Additional bands at : 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
We recommend blocking with 1% milk.
References for Anti-YAP1 antibody (ab39361)
This product has been referenced in:
Beverdam A et al. Yap Controls Stem/Progenitor Cell Proliferation in the Mouse Postnatal Epidermis. J Invest Dermatol : (2012).
Read more (PubMed: 23190885) »