Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab56701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 49 kDa.
IHC-P Use a concentration of 3 µg/ml.

Target

  • Function
    Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).
  • Tissue specificity
    Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
  • Sequence similarities
    Belongs to the YORKIE family.
    Contains 2 WW domains.
  • Post-translational
    modifications
    Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage.
  • Cellular localization
    Cytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density.
  • Information by UniProt
  • Database links
  • Alternative names
    • 65 kDa Yes associated protein antibody
    • 65 kDa Yes-associated protein antibody
    • COB1 antibody
    • YAp 1 antibody
    • YAP 65 antibody
    • YAP antibody
    • YAP1 antibody
    • YAP1_HUMAN antibody
    • YAP2 antibody
    • YAP65 antibody
    • yes -associated protein delta antibody
    • Yes associated protein 1 65kDa antibody
    • Yes associated protein 1 antibody
    • Yes associated protein 2 antibody
    • yes associated protein beta antibody
    • YKI antibody
    • Yorkie homolog antibody
    see all

Images



  • Predicted band size : 49 kDa
    YAP1 antibody (ab56701) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • YAP1 antibody (ab56701) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium.

References

This product has been referenced in:

See all 9 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HEK293T
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 14 2017

Application
Immunocytochemistry
Sample
Human Cultured Cells (immortalised glioma cell lines U343 and U87)
Permeabilization
Yes - 0.1% Triton-X-100
Specification
immortalised glioma cell lines U343 and U87
Fixative
Paraformaldehyde
Username

Katherine Eales

Verified customer

Submitted Nov 30 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (oocyte)
Gel Running Conditions
Reduced Denaturing (7,5)
Loading amount
20 µg
Specification
oocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 27 2016

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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