Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab62752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

Target

  • FunctionTranscriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).
  • Tissue specificityIncreased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
  • Sequence similaritiesBelongs to the YORKIE family.
    Contains 2 WW domains.
  • Post-translational
    modifications
    Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage.
  • Cellular localizationCytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density.
  • Information by UniProt
  • Database links
  • Alternative names
    • 65 kDa Yes associated protein antibody
    • 65 kDa Yes-associated protein antibody
    • COB1 antibody
    • YAp 1 antibody
    • YAP 65 antibody
    • YAP antibody
    • YAP1 antibody
    • YAP1_HUMAN antibody
    • YAP2 antibody
    • YAP65 antibody
    • yes -associated protein delta antibody
    • Yes associated protein 1 65kDa antibody
    • Yes associated protein 1 antibody
    • Yes associated protein 2 antibody
    • yes associated protein beta antibody
    • YKI antibody
    • Yorkie homolog antibody
    see all

Anti-YAP1 antibody images

  • All lanes : Anti-YAP1 antibody (ab62752) at 1/2000 dilution

    Lane 1 : Whole cell lysates of HEK-293T cells co-transfected with human c-Abl and human YAP1
    Lane 2 : Whole cell lysates of untransfected HEK-293T
    Lane 3 : Whole cell lysates of HEK-293T cells co-transfected with human c-Abl and human YAP1 with YAP1 immunizing peptide (human 442-454) at 20 µg/ml

    Secondary
    Goat Anti-Rabbit IgG, Peroxidase conjugate

    Predicted band size : 65 kDa
    Observed band size : 65 kDa

References for Anti-YAP1 antibody (ab62752)

ab62752 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"