Overview

  • Product nameAnti-YAP1 (phospho Y357) antibody
    See all YAP1 primary antibodies
  • Description
    Rabbit polyclonal to YAP1 (phospho Y357)
  • Specificityab62751 specifically recognizes human phospho-YAP1 (phospho Y357). Staining of the phospho-YAP1 (phospho Y357) band in Western blotting is specifically inhibited by the immunizing peptide. No inhibition with the unmodified peptide is observed.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish, Opossum
  • Immunogen

    Synthetic phosphopeptide: GLSMSSYPSVPRT conjugated to KLH, corresponding to C-terminal amino acids 351-362 (phosho Y357) of Human YAP1.

  • Positive control
    • HEK-293T cells co-transfected with human YAP1 and human c-Abl.

Properties

Applications

Our Abpromise guarantee covers the use of ab62751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
ICC/IF Use a concentration of 5 - 10 µg/ml.

Target

  • FunctionTranscriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).
  • Tissue specificityIncreased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
  • Sequence similaritiesBelongs to the YORKIE family.
    Contains 2 WW domains.
  • Post-translational
    modifications
    Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage.
  • Cellular localizationCytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density.
  • Information by UniProt
  • Database links
  • Alternative names
    • 65 kDa Yes associated protein antibody
    • 65 kDa Yes-associated protein antibody
    • COB1 antibody
    • YAp 1 antibody
    • YAP 65 antibody
    • YAP antibody
    • YAP1 antibody
    • YAP1_HUMAN antibody
    • YAP2 antibody
    • YAP65 antibody
    • yes -associated protein delta antibody
    • Yes associated protein 1 65kDa antibody
    • Yes associated protein 1 antibody
    • Yes associated protein 2 antibody
    • yes associated protein beta antibody
    • YKI antibody
    • Yorkie homolog antibody
    see all

Anti-YAP1 (phospho Y357) antibody images

  • ab62751 staining YAP1 (phospho Y357) in rat oligodendrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% normal goat serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/300 in PBS + 10% normal goat serum) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-YAP1 (phospho Y357) antibody (ab62751) at 1/2000 dilution

    Lane 1 : Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl
    Lane 2 : Whole cell lysates of untransfected HEK-293T cells
    Lane 3 : Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl with YAP1 peptide (human 351-362) at 20 µg/ml
    Lane 4 : Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl with phospho-YAP1 (human 351-362 [phospho Y357]) immunizing peptide at 20 µg/ml

    Secondary
    Goat Anti-Rabbit IgG, Peroxidase conjugate

    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 100 kDa,70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-YAP1 (phospho Y357) antibody (ab62751)

This product has been referenced in:
  • Pijuan-Galitó S  et al. Serum Inter-a-inhibitor activates the Yes tyrosine kinase and YAP/TEAD transcriptional complex in mouse embryonic stem cells. J Biol Chem 289:33492-502 (2014). Read more (PubMed: 25301940) »
  • Liu X  et al. PTPN14 interacts with and negatively regulates the oncogenic function of YAP. Oncogene : (2012). Read more (PubMed: 22525271) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Rat Cell (oligodendrocytes)
Specification oligodendrocytes
Permeabilization Yes - 0,1% Triton-X 100
Fixative Paraformaldehyde
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Submitted Sep 24 2014

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Antibodies generally cross react with proteins of different species if the protein shares sequence homology. The homology must be >84%. We at Abcam use the terminology "predicted to react" based on thi...

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