• Product nameAnti-YB1 antibody
    See all YB1 primary antibodies
  • Description
    Rabbit polyclonal to YB1
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pigeon, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 224-274 of Human YB1 (NP_004550).

  • Positive control
    • Hela, Jurkat and 293T cell lysates



Our Abpromise guarantee covers the use of ab114999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 36 kDa.
IP Use at 2-10 µg/mg of lysate.


  • FunctionMediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.
    The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation.
  • Sequence similaritiesContains 1 CSD (cold-shock) domain.
  • Post-translational
    Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcativational ability.
    In the absence of phosphorylation the protein is retained in the cytoplasm.
    Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic granule. Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.
  • Information by UniProt
  • Database links
  • Alternative names
    • BP 8 antibody
    • CBF-A antibody
    • CCAAT binding transcription factor I subunit A antibody
    • CCAAT-binding transcription factor I subunit A antibody
    • CSDA2 antibody
    • CSDB antibody
    • DBPB antibody
    • DNA binding protein B antibody
    • DNA-binding protein B antibody
    • EFI-A antibody
    • Enhancer factor I subunit A antibody
    • MDR NF1 antibody
    • MGC104858 antibody
    • MGC110976 antibody
    • MGC117250 antibody
    • NSEP 1 antibody
    • NSEP1 antibody
    • Nuclease sensitive element binding protein 1 antibody
    • Nuclease-sensitive element-binding protein 1 antibody
    • p50 antibody
    • Q15905 antibody
    • Y-box binding protein 1 antibody
    • Y-box transcription factor antibody
    • Y-box-binding protein 1 antibody
    • YB 1 antibody
    • YB-1 antibody
    • YBOX1_HUMAN antibody
    • YBX 1 antibody
    • ybx1 antibody
    see all

Anti-YB1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling YB1 with ab114999 at 1/1000 (1µg/ml). Detection: DAB.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach linitis plastica tissue labelling YB1 with ab114999 at 1/5000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-YB1 antibody (ab114999) at 0.1 µg/ml

    Lane 1 : Hela cell lysate at 50 µg
    Lane 2 : Hela cell lysate at 15 µg
    Lane 3 : 293T cell lysate at 50 µg
    Lane 4 : Jurkat cell lysate at 50 µg

    developed using the ECL technique

    Predicted band size : 36 kDa

    Exposure time : 30 seconds
  • Immunoprecipitation : 1 mg of Hela cell lysate immunoprecipitated using ab11499 at 6 µg/mg lysate; 20% of immunoprecipitate loaded per lane

    Western Blot: ab114999 at 1 µg/ml

    developed using the ECL technique

    Exposure time: 3 seconds

    Predicted band size : 36 kDa

References for Anti-YB1 antibody (ab114999)

ab114999 has not yet been referenced specifically in any publications.

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