Anti-YB1 antibody - Aminoterminal end (ab71557)

Overview

  • Product nameAnti-YB1 antibody - Aminoterminal end
    See all YB1 primary antibodies
  • Description
    Rabbit polyclonal to YB1 - Aminoterminal end
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the N terminal region of human YB1.

  • Positive control
    • NCI-H460 cell line lysates and YB1 transfected 293 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab71557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 32 kDa (predicted molecular weight: 36 kDa).
ELISA 1/1000.

Target

  • FunctionMediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.
    The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation.
  • Sequence similaritiesContains 1 CSD (cold-shock) domain.
  • Post-translational
    modifications
    Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcativational ability.
    In the absence of phosphorylation the protein is retained in the cytoplasm.
    Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic granule. Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.
  • Information by UniProt
  • Database links
  • Alternative names
    • BP 8 antibody
    • CBF-A antibody
    • CCAAT binding transcription factor I subunit A antibody
    • CCAAT-binding transcription factor I subunit A antibody
    • CSDA2 antibody
    • CSDB antibody
    • DBPB antibody
    • DNA binding protein B antibody
    • DNA-binding protein B antibody
    • EFI-A antibody
    • Enhancer factor I subunit A antibody
    • MDR NF1 antibody
    • MGC104858 antibody
    • MGC110976 antibody
    • MGC117250 antibody
    • NSEP 1 antibody
    • NSEP1 antibody
    • Nuclease sensitive element binding protein 1 antibody
    • Nuclease-sensitive element-binding protein 1 antibody
    • p50 antibody
    • Q15905 antibody
    • Y-box binding protein 1 antibody
    • Y-box transcription factor antibody
    • Y-box-binding protein 1 antibody
    • YB 1 antibody
    • YB-1 antibody
    • YBOX1_HUMAN antibody
    • YBX 1 antibody
    • ybx1 antibody
    see all

Anti-YB1 antibody - Aminoterminal end images

  • Anti-YB1 antibody - Aminoterminal end (ab71557) at 1/60 dilution + NCI-H460 cell line lysate at 30 µg

    Predicted band size : 36 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 17 kDa,20 kDa,25 kDa,57 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-YB1 antibody - Aminoterminal end (ab71557) at 1/60 dilution

    Lane 1 : untransfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the YBX1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 36 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-YB1 antibody - Aminoterminal end (ab71557)

ab71557 has not yet been referenced specifically in any publications.

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