Anti-YB1 antibody [EP2706Y] (ab76540)

Overview

  • Product nameAnti-YB1 antibody [EP2706Y]
    See all YB1 primary antibodies
  • Description
    Rabbit monoclonal [EP2706Y] to YB1
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N terminus of human YB1

  • Positive control
    • HeLa, 293T, Jurkat and MCF7 cell lysates; permeabilized HeLa cells.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab76540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Predicted molecular weight: 36 kDa.
IP 1/50.
Flow Cyt 1/60. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionMediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.
      The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation.
    • Sequence similaritiesContains 1 CSD (cold-shock) domain.
    • Post-translational
      modifications
      Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcativational ability.
      In the absence of phosphorylation the protein is retained in the cytoplasm.
      Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus.
    • Cellular localizationCytoplasm. Nucleus. Cytoplasmic granule. Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.
    • Information by UniProt
    • Database links
    • Alternative names
      • BP 8 antibody
      • CBF-A antibody
      • CCAAT binding transcription factor I subunit A antibody
      • CCAAT-binding transcription factor I subunit A antibody
      • CSDA2 antibody
      • CSDB antibody
      • DBPB antibody
      • DNA binding protein B antibody
      • DNA-binding protein B antibody
      • EFI-A antibody
      • Enhancer factor I subunit A antibody
      • MDR NF1 antibody
      • MGC104858 antibody
      • MGC110976 antibody
      • MGC117250 antibody
      • NSEP 1 antibody
      • NSEP1 antibody
      • Nuclease sensitive element binding protein 1 antibody
      • Nuclease-sensitive element-binding protein 1 antibody
      • p50 antibody
      • Q15905 antibody
      • Y-box binding protein 1 antibody
      • Y-box transcription factor antibody
      • Y-box-binding protein 1 antibody
      • YB 1 antibody
      • YB-1 antibody
      • YBOX1_HUMAN antibody
      • YBX 1 antibody
      • ybx1 antibody
      see all

    Anti-YB1 antibody [EP2706Y] images

    • Flow cytometric analysis of permeabilized HeLa cells using ab76540 at 1/60 dilution (red) or a rabbit IgG (negative) (green)
    • All lanes : Anti-YB1 antibody [EP2706Y] (ab76540) at 1/20000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 36 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)

    References for Anti-YB1 antibody [EP2706Y] (ab76540)

    ab76540 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (293T)
    Loading amount 30000 cells
    Specification 293T
    Gel Running Conditions Reduced Denaturing (4-20% TrisGlycine)
    Blocking step 5% Milk, 1% BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    Submitted Feb 24 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"