Overview

  • Product nameAnti-YBEY antibody
  • Description
    Rabbit polyclonal to YBEY
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: DDYNLGDIFL GVEYIFHQCK ENEDYNDVLT VTATHGLCHL LGFTHGTEAE WQQMFQKEKA VLDELGRRTG TRLQPLT, corresponding to amino acids 84-160 of Human YBEY.

  • Positive control
    • Human thyroid gland tissue; RT 4 and U -251 MG cell lysates; Human Liver and Tonsil lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • FunctionMay have metal-dependent hydrolase activity.
  • Sequence similaritiesBelongs to the rRNA maturation factor YbeY family.
  • Information by UniProt
  • Database links
  • Alternative names
    • C21orf57 antibody
    • Putative metalloprotease C21orf57 antibody
    • rRNA maturation factor homolog antibody
    • ybeY antibody
    • YbeY metallopeptidase antibody
    • YBEY_HUMAN antibody
    see all

Anti-YBEY antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab121504 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121504, at 1/400 dilution, staining YBEY in paraffin-embedded Human thyroid gland tissue by Immunohistochemistry.
  • All lanes : Anti-YBEY antibody (ab121504) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

References for Anti-YBEY antibody (ab121504)

ab121504 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"