Overview

  • Product nameAnti-Yersinia pestis antibody [YPF19]
  • Description
    Mouse monoclonal [YPF19] to Yersinia pestis
  • SpecificityThis antibody recognises Y.pestis F1 capsular antigen. There is no cross-reactivity with Y.pseudotuberculosis and Y. enterocolitica.
  • Tested applicationsSuitable for: IHC-Fr, WB, ELISAmore details
  • Immunogen

    Purified F1 antigen from Yersinia pestis vaccine strain EV76.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberYPF19
  • MyelomaSp2/0
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • RelevanceY. pestis has a number of virulence factors that enable it to survive in humans by facilitating use of host nutrients, causing damage to host cells, and subverting phagocytosis and other host defense mechanisms. Capsular F1 antigen is a main immunochemical component of Y. pestis surface. Its synthesis is determined by pFra plasmid and induced at 370C. F1 is aggregated on the outer membrane of the Y. pestis microbe as an olygomeric protein, forming a granular layer and gradually diffusing into the environment. Capsular polymer consists of multiple similar hydrophobic protein subunits, aggregating at physiological conditions. F1 subunit of the antigen is originally synthesized from 170 amino acids and has a molecular mass 17.6 K, then, after the elimination of the signal peptide during the secretion onto the surface, a protein with molecular mass 15.6 K and isoelectric point 4.1 is formed.
  • Cellular localizationCell Membrane
  • Alternative names
    • Y pestis antibody
    • Y. pestis antibody
    • Y.pestis antibody

References for Anti-Yersinia pestis antibody [YPF19] (ab8275)

This product has been referenced in:
  • Ye Z  et al. Caspase-3 mediates the pathogenic effect of Yersinia pestis YopM in liver of C57BL/6 mice and contributes to YopM's function in spleen. PLoS One 9:e110956 (2014). IHC ; Mouse . Read more (PubMed: 25372388) »

See 1 Publication for this product

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I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Unfortunately we have no information concerning this question, because we have not determined that in our laboratory.

This is a very new product, and I'm afriad all the information we have is on the datasheet. The anitbody has not yet been tested in Western Blotting. A standard protocol for WB is available in the "Protocols" section of the Abcam website

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"