Anti-Yersinia pestis F1 antigen antibody [YPF19] (ab20833)

Overview

  • Product nameAnti-Yersinia pestis F1 antigen antibody [YPF19]
    See all Yersinia pestis F1 antigen primary antibodies
  • Description
    Mouse monoclonal [YPF19] to Yersinia pestis F1 antigen
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with Y. pestis. Does not react with Y. pseudotuberculosis and Y. enterocolitica. Not yet tested in otehr species.
  • Immunogen

    Purified F1 antigen from Y. pestis vaccine strain EB

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notes>95% pure. Purity is tested by electrophoresis
  • ClonalityMonoclonal
  • Clone numberYPF19
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 18 kDa. Dilution optimised using Chromogenic detection.
ICC/IF Use at an assay dependent dilution.

Target

  • RelevanceYersinia pestis is a gram-negative coccobacillus belonging to the Enterobacteriaceae. Yersinia pestis is primarily a rodent pathogen, with humans being an accidental host when bitten by an infected rat flea.  The flea draws viable Y. pestis organisms into its intestinal tract. Some Y. pestis in the flea are then regurgitated when the flea gets its blood meal thus transferring the infection to a new host. While growing in the flea, Y. pestis loses its capsular layer, which is made of the F1 (or fraction 1) capsule antigen. Most of the organisms are phagocytosed and killed by the polymorphonuclear leukocytes in the human host. A few bacilli are taken up by tissue macrophages. The macrophages are unable to kill Y. pestis and provide a protected environment for the organisms to synthesize their virulence factors.
  • Cellular localizationSecreted
  • Alternative names
    • Caf1 antibody
    • F1 capsule antigen antibody

References for Anti-Yersinia pestis F1 antigen antibody [YPF19] (ab20833)

ab20833 has not yet been referenced specifically in any publications.

Product Wall

Thank you for the details that you have provided and I'm sorry to hear that you are experiencing difficulty with ab20833. The antibody should absolutely detect purified Yersinia pestis F1 protein. I just need to check a few points; do you know that the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"