Overview

  • Product nameAnti-YIPF3 antibody
    See all YIPF3 primary antibodies
  • Description
    Rabbit polyclonal to YIPF3
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 302-350 (GILDTLEGPN IPPIQRVPRD IPAMLPAARL PTTVLNATAK AVAVTLQSH) of Human YIPF3.

  • Positive control
    • Transfected HEK293T Over-expression Lysate; Human colon tissue; A431 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab121456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500.
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • FunctionMay play a role in hematopoiesis.
  • Tissue specificityExpressed by nucleated hematopoietic cells (at protein level).
  • Sequence similaritiesBelongs to the YIP1 family.
  • Developmental stageExpressed in fetal liver (at protein level). Expressed in embryonic hematopoiesis sites.
  • Post-translational
    modifications
    Not glycosylated.
  • Cellular localizationCell membrane. Cytoplasm. Localization to the cytoplasm or to the cell membrane is developmentally and ontogenetically regulated.
  • Information by UniProt
  • Database links
  • Alternative names
    • Killer lineage protein 1 antibody
    • Natural killer cell-specific antigen KLIP1 antibody
    • Protein YIPF3 antibody
    • YIP1 family member 3 antibody
    • Yipf3 antibody
    • YIPF3_HUMAN antibody
    see all

Anti-YIPF3 antibody images

  • All lanes : Anti-YIPF3 antibody (ab121456) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : YIPF3-transfected HEK293T over expression Lysate (co-expressed with a C terminal myc-DDK tag (~3.1 kDa))

    Developed using the ECL technique
  • ab121456, at a 1/15 dilution, staining YIPF3 in paraffin embedded Human colon tissue by Immunohistochemistry.
  • ab121456, at 4 µg/ml, staining YIPF3 (green) in Human A431 cells by Immunofluorescence.

References for Anti-YIPF3 antibody (ab121456)

ab121456 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"