Anti-YIPF6 antibody (ab80442)


  • Product nameAnti-YIPF6 antibody
  • Description
    Rabbit polyclonal to YIPF6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Xenopus laevis
  • Immunogen

    Synthetic peptide: NFMVRLFVVI VMFAWSIVAS TALLADSQPP NRRALAVYPV FLFYFVISWM , corresponding to C terminal amino acids 179-228 of Human YIPF6

  • Positive control
    • Fetal muscle lysate


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab80442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


Anti-YIPF6 antibody images

  • Anti-YIPF6 antibody (ab80442) at 1 µg/ml + Fetal muscle lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
  • ab80442 staining YIPF6 in 293 Human embryonic kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% saponin and blocked with 10% serum for 2 hours at 23°C. Samples were incubated with primary antibody (1/500 in 0.5% saponin) for 2 hours at 23°C. An Alexa Fluor®555-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-YIPF6 antibody (ab80442)

ab80442 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for taking the time to send us the questionnaire and the image attached.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of qualit...

Read More

Thank you for your reply. I am happy the information provided was helpful to you.

I have issued a credit note for the value of the antibody ab80442.

Your credit note ID is XXXXX.

The credit note may be used in ...

Read More

I just wanted to update you with the last details provided by my lab colleagues regarding the use of this antibody in different cell lines and tissues.

They just confirmed they have unsuccessfully tested it on a few human cell lines (HepG2, J...

Read More

Thank you for your reply.

We haven’t had any other complaints from customers using this antibody in WB or IHC, so unfortunately I am unable to inform you about its use in other labs.

My lab colleagues confirmed they have never...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (293 human embryonic kidney)
Specification 293 human embryonic kidney
Fixative Formaldehyde
Permeabilization Yes - 0,5%(w/v) saponin
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jul 21 2010