Overview

  • Product nameAnti-YKT6 antibody
  • Description
    Mouse monoclonal to YKT6
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Human, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment (proprietary-tag) corresponding to Human YKT6 aa 99-198.
    Sequence:

    DEFSKQVDRIDWPVGSPATIHYPALDGHLSRYQNPREADPMTKVQAELDE TKIILHNTMESLLERGEKLDDLVSKSEVLGTQSKAFYKTARKQNSCCAIM


    Database link: NP_006546.1

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 22 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionVesicular soluble NSF attachment protein receptor (v-SNARE) mediating vesicle docking and fusion to a specific acceptor cellular compartment. Functions in endoplasmic reticulum to Golgi transport; as part of a SNARE complex composed of GOSR1, GOSR2 and STX5. Functions in early/recycling endosome to TGN transport; as part of a SNARE complex composed of BET1L, GOSR1 and STX5. Has a S-palmitoyl transferase activity.
  • Sequence similaritiesBelongs to the synaptobrevin family.
    Contains 1 longin domain.
    Contains 1 v-SNARE coiled-coil homology domain.
  • DomainThe longin domain regulates palmitoylation and membrane targeting.
  • Post-translational
    modifications
    Palmitoylated; catalyzes its own palmitoylation. Palmitoylation is required for Golgi targeting.
    Farnesylation is required for Golgi targeting.
  • Cellular localizationCytoplasm > cytosol. Cytoplasmic vesicle membrane. Golgi apparatus membrane. Probably cycles through vesicles between Golgi and endosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • R SNARE antibody
    • SNARE protein Ykt6 antibody
    • Synaptobrevin homolog YKT6 antibody
    • YKT 6 antibody
    • YKT6 antibody
    • YKT6 S. cerevisiae homolog of antibody
    • YKT6 v SNARE homolog antibody
    • YKT6 v SNARE protein antibody
    • YKT6_HUMAN antibody
    see all

Anti-YKT6 antibody images

  • Anti-YKT6 antibody (ab77150) at 5 µg/ml + immunogen (GST-tagged 100 aa recombinant fragment) at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

    Predicted band size : 22 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-YKT6 antibody (ab77150)

ab77150 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (primary neuron cell)
Gel Running Conditions Reduced Denaturing (13%)
Loading amount 50 µg
Specification primary neuron cell
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Jie Zhang

Verified customer

Submitted Jul 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"