Overview

  • Product name
  • Description
    Mouse polyclonal to YL1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Orangutan
  • Immunogen

    Recombinant full length protein corresponding to amino acids 1-364 of Human YL1 (NP_005988.1).

  • Positive control
    • YL1 transfected 293T cell line; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Constituent: 100% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Could be a DNA-binding transcriptional regulator. May be involved in chromatin modification and remodeling.
  • Sequence similarities
    Belongs to the VPS72/YL1 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFL1 antibody
    • HGNC11644 antibody
    • Protein YL-1 antibody
    • Swc2 antibody
    • TCFL1 antibody
    • Transcription factor like 1 (YL1) antibody
    • Transcription Factor Like 1 antibody
    • Transcription factor-like 1 antibody
    • Vacuolar protein sorting 72 (yeast) antibody
    • Vacuolar protein sorting 72 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting associated protein 72 homolog antibody
    • Vacuolar protein sorting-associated protein 72 homolog antibody
    • VPS72 antibody
    • VPS72_HUMAN antibody
    • YL-1 antibody
    • YL1 antibody
    see all

Images

  • Immunofluorescence analysis of HeLa cells, labeling YL1 with ab167639 10 µg/ml
  • All lanes : Anti-YL1 antibody (ab167639) at 1 µg/ml

    Lane 1 : YL1 transfected 293T cell line
    Lane 2 : Non-transfected 293T cell line

    Lysates/proteins at 15 µl per lane.


    Predicted band size : 41 kDa

References

ab167639 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (Kidney cell line)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
3000 cells
Specification
Kidney cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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Verified customer

Submitted Nov 30 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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