Customer reviews and FAQs

Overview

  • Product name
    Anti-YL1 antibody
    See all YL1 primary antibodies
  • Description
    Rabbit polyclonal to YL1
  • Specificity
    Detects endogenous levels of total YL1 protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal region of human YL1

  • Positive control
    • Human breast carcinoma tissue RAW264.7 cell extract NIH/3T3 cell extract

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Could be a DNA-binding transcriptional regulator. May be involved in chromatin modification and remodeling.
  • Sequence similarities
    Belongs to the VPS72/YL1 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • CFL1 antibody
      • HGNC11644 antibody
      • Protein YL-1 antibody
      • Swc2 antibody
      • TCFL1 antibody
      • Transcription factor like 1 (YL1) antibody
      • Transcription Factor Like 1 antibody
      • Transcription factor-like 1 antibody
      • Vacuolar protein sorting 72 (yeast) antibody
      • Vacuolar protein sorting 72 homolog (S. cerevisiae) antibody
      • Vacuolar protein sorting associated protein 72 homolog antibody
      • Vacuolar protein sorting-associated protein 72 homolog antibody
      • VPS72 antibody
      • VPS72_HUMAN antibody
      • YL-1 antibody
      • YL1 antibody
      see all

    Anti-YL1 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - YL1 antibody (ab72506)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - YL1 antibody (ab72506)
      Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab72506 at 1/50 dilution. Right panel was also treated with immunising peptide.
    • Western blot - YL1 antibody (ab72506)
      Western blot - YL1 antibody (ab72506)
      All lanes : Anti-YL1 antibody (ab72506) at 1/500 dilution

      Lane 1 : RAW264.7 cell extract
      Lane 2 : NIH 3T3 cell extract
      Lane 3 : NIH 3T3 cell extract with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 41 kDa
      Observed band size : 41 kDa
      Additional bands at : 30 kDa,48 kDa,50 kDa,76 kDa,78 kDa,87 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-YL1 antibody (ab72506)

    ab72506 has not yet been referenced specifically in any publications.

    Publishing research using ab72506? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

    Submit an abreview for ab72506 Submit a question for ab72506

    1 Abreview or Q&A

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    Western blot abreview for Anti-YL1 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Kidney cell line)
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Loading amount
    3000 cells
    Specification
    Kidney cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    Verified customer

    Submitted Dec 06 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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