Overview

  • Product name
  • Description
    Rabbit polyclonal to YL1
  • Specificity
    Detects endogenous levels of total YL1 protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal region of human YL1

  • Positive control
    • Human breast carcinoma tissue RAW264.7 cell extract NIH/3T3 cell extract

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Could be a DNA-binding transcriptional regulator. May be involved in chromatin modification and remodeling.
  • Sequence similarities
    Belongs to the VPS72/YL1 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFL1 antibody
    • HGNC11644 antibody
    • Protein YL-1 antibody
    • Swc2 antibody
    • TCFL1 antibody
    • Transcription factor like 1 (YL1) antibody
    • Transcription Factor Like 1 antibody
    • Transcription factor-like 1 antibody
    • Vacuolar protein sorting 72 (yeast) antibody
    • Vacuolar protein sorting 72 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting associated protein 72 homolog antibody
    • Vacuolar protein sorting-associated protein 72 homolog antibody
    • VPS72 antibody
    • VPS72_HUMAN antibody
    • YL-1 antibody
    • YL1 antibody
    see all

Anti-YL1 antibody images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab72506 at 1/50 dilution. Right panel was also treated with immunising peptide.
  • All lanes : Anti-YL1 antibody (ab72506) at 1/500 dilution

    Lane 1 : RAW264.7 cell extract
    Lane 2 : NIH 3T3 cell extract
    Lane 3 : NIH 3T3 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 30 kDa,48 kDa,50 kDa,76 kDa,78 kDa,87 kDa. We are unsure as to the identity of these extra bands.

References for Anti-YL1 antibody (ab72506)

ab72506 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (Kidney cell line)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
3000 cells
Specification
Kidney cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Dec 06 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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