Overview

  • Product name
  • Description
    Rabbit polyclonal to YOD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: DMLIIEEDQT RPRSSPAFTK RGASSYVRET LPVLTRTVVP ADNSCLFTSV YYVVEGGVLN PACAPEMRRL IAQIVA, corresponding to amino acids 116-191 of Human YOD1.

  • Positive control
    • Human colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Able to cleave both polyubiquitin and di-ubiquitin.
  • Sequence similarities
    Contains 1 C2H2-type zinc finger.
    Contains 1 OTU domain.
  • Domain
    The UBAX-like region mediates the interaction with VCP. According to PubMed:19818707, it corresponds to a UBX domain, which is a hallmark for VCP-associated proteins. However, no canonical UBX is detected by prediction tools such as Pfam or PROSITE.
  • Information by UniProt
  • Database links
  • Alternative names
    • YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae) edit item name - YOD1 OTU deubiquinating enzyme 1, S. cerevisiae, homolog of antibody
    • DUBA-8 antibody
    • DUBA8 antibody
    • HIN-7 antibody
    • HIV-1-induced protease 7 antibody
    • HsHIN7 antibody
    • OTU domain containing 2 antibody
    • OTU domain-containing protein 2 antibody
    • OTU1_HUMAN antibody
    • OTUD2 antibody
    • PRO0907 antibody
    • RP11-164O23.1 antibody
    • Ubiquitin thioesterase OTU1 antibody
    • yod1 antibody
    • YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae) antibody
    • YOD1 OTU deubiquinating enzyme 1 homolog (yeast) antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, plasma membrane and cytoskeleton (actin filaments). Recommended concentration of ab121984 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121984, at 1/400 dilution, staining YOD1 in Paraffin-embedded Human colon tissue by Immunohistochemistry.

References

ab121984 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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