Overview

  • Product nameAnti-YTHDC1 antibody
    See all YTHDC1 primary antibodies
  • Description
    Rabbit polyclonal to YTHDC1
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to internal sequence amino acids 69-160 (LVSKPLSSSV SNNKRIVSTK GKSATEYKNE EYQRSERNKR LDADRKIRLS SSASREPYKN QPEKTCVRKR DPERRAKSPT PDGSERIGLE VD) of Human YTHDC1

  • Positive control
    • Human esophagus tissue; RT-4 and U-251 MG cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab122340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • FunctionMay be part of a signal transduction pathway that influences splice site selection.
  • Sequence similaritiesContains 1 YTH domain.
  • Cellular localizationNucleus. Localizes to a novel subnuclear structure, the YT bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1966 antibody
    • Putative splicing factor YT521 antibody
    • splicing factor YT521-B antibody
    • YT521 antibody
    • YT521-B antibody
    • YTDC1_HUMAN antibody
    • YTH domain-containing protein 1 antibody
    • YTHDC1 antibody
    see all

Anti-YTHDC1 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and plasma membrane. Recommended concentration of ab122340 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122340, at a 1/400 dilution, staining YTHDC1 in paraffin-embedded Human esophagus tissue by immunohistochemistry.
  • All lanes : Anti-YTHDC1 antibody (ab122340) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique
  • Immunohistochemical staining of Human fallopian tube shows strong nuclear positivity in glandular cells using ab122340

References for Anti-YTHDC1 antibody (ab122340)

ab122340 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa soluble lysate)
Total protein in input 2e+007 cells
Immuno-precipitation step Protein A
Specification HeLa soluble lysate
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Abcam user community

Verified customer

Submitted Nov 29 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Gel)
Loading amount 100 µg
Treatment Knockdown verification
Specification HeLa
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Nov 18 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"