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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-YY1 antibody - ChIP Grade
See all YY1 products (9) ...
Description
Rabbit polyclonal to YY1 - ChIP Grade
Specificity
ab12132 reacts with human YY1.
Tested applications
ChIP, ICC/IF, WB, EMSAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Monkey
Immunogen
Synthetic peptide, corresponding to amino acids 109-123 of Human YY1.
Positive control
Raji nuclear extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide, 0.01% Thimerosal (merthiolate)
Constituents: 0.2% Gelatin, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
Affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution. (PubMed: 20106920)
ICC/IF: Use a concentration of 1 - 5 µg/ml.
WB: 1/500 - 1/1000.Detects a band of approximately 63 kDa (predicted molecular weight: 49.7 kDa).(For optimal results, primary antibody incubations should be performed at 4°C.)
EMSA: Use at an assay dependent concentration. (PubMed: 22210889)
Target
Function
Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
Sequence similarities
Belongs to the YY transcription factor family.
Contains 4 C2H2-type zinc fingers.
Cellular localization
Nucleus matrix. Associated with the nuclear matrix.
Target information above from: UniProt accessionP25490
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- YY1 transcription factor antibody
- CF1 antibody
- Delta antibody
see all
Database links
- Entrez Gene: 7528 Human
- Entrez Gene: 22632 Mouse
- Entrez Gene: 24919 Rat
- Omim: 600013 Human
- SwissProt: P25490 Human
- SwissProt: Q00899 Mouse
- Unigene: 388927 Human
- Unigene: 3868 Mouse
see all
Anti-YY1 antibody - ChIP Grade images:
Western blot - YY1 antibody (ab12132)
Predicted band size : 49.7 kDa
Detection of YY1 by Western blot analysis. YY1 can be detected in nuclear extracts derived from Raji cells using ab12132 (lane 1) at a 1:1000 dilution. The signal corresponding to nuclear YY1 is eliminated by adding the immunizing peptide to the reaction mixture (lane 2).
Immunocytochemistry/ Immunofluorescence-YY1 antibody(ab12132)
ICC/IF image of ab12132 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12132, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-YY1 antibody - ChIP Grade (ab12132)
This product has been referenced in:
- Golan-Mashiach Met al. Identification of CTCF as a master regulator of the clustered protocadherin genes. Nucleic Acids Res : (2011). EMSA.Read more (PubMed: 22210889) »
- Liu XFet al. Biphasic recruitment of transcriptional repressors to the murine cytomegalovirus major immediate-early promoter during the course of infection in vivo. J Virol 84:3631-43 (2010). ChIP; Mouse.Read more (PubMed: 20106920) »
See all 6 publications for this product
Publishing research using ab12132? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"