Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab38422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 19721712
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

Target

  • FunctionMultifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
  • Sequence similaritiesBelongs to the YY transcription factor family.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localizationNucleus matrix. Associated with the nuclear matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CF1 antibody
    • Delta antibody
    • Delta transcription factor antibody
    • INO80 complex subunit S antibody
    • INO80S antibody
    • NF E1 antibody
    • NF-E1 antibody
    • NFE1 antibody
    • OTTHUMP00000197459 antibody
    • Transcriptional repressor protein YY1 antibody
    • TYY1_HUMAN antibody
    • UCR motif DNA binding protein antibody
    • UCRBP antibody
    • Yin and yang 1 antibody
    • Yin and Yang 1 protein antibody
    • Yin Yang 1 antibody
    • Ying Yang 1 antibody
    • YY 1 antibody
    • YY 1 transcription factor antibody
    • YY-1 antibody
    • YY1 antibody
    • YY1 transcription factor antibody
    see all

Anti-YY1 antibody - ChIP Grade images

  • All lanes : Anti-YY1 antibody - ChIP Grade (ab38422) at 1/100 dilution

    Lane 1 : HL60 cell lysate
    Lane 2 : Mouse kidney tissue lysate


    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 30 kDa,65 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of YY1 expression in formalin-fixed and paraffin-embedded human hepatocarcinoma tissue using 1/50 ab38422.

References for Anti-YY1 antibody - ChIP Grade (ab38422)

This product has been referenced in:
  • Guaraldo M  et al. Characterization of human mitochondrial ferritin promoter: identification of transcription factors and evidences of epigenetic control. Sci Rep 6:33432 (2016). ChIP ; Human . Read more (PubMed: 27625068) »
  • Song G & Wang L Nuclear receptor SHP activates miR-206 expression via a cascade dual inhibitory mechanism. PLoS One 4:e6880 (2009). ChIP . Read more (PubMed: 19721712) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. Unfortunately none of our YY1 antibodies has been tested in ChIP therefore we do not know what suitable genes would be a good positive control. I suggest looking in the literature on this protein and hope this will be able t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"