|
|
|
|
Western blot - YY1 antibody (ab38422)
(enlarge)
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - YY1 antibody (ab38422)
(enlarge)
|
|
|
|
|
Product Name
|
YY1 antibody - ChIP Grade
|
| |
See all YY1 antibodies (7)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to YY1 - ChIP Grade
|
|
Immunogen
|
KLH-conjugated synthetic peptide selected from the region of aa 250-300 of human YY1 protein.
|
|
Reacts with
(species key)
|
Hu, Ms
|
|
Tested applications
(see key)
|
ChIP, ELISA, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ChIP: Use at an assay dependent dilution (see PubMed 19721712).
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
human hepatocarcinoma tissue. HL60 cell lysate. mouse kidney tissue lysate
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
|
|
Relevance
|
YY1 is a ubiquitously distributed transcription factor belonging to the GLI Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.
|
|
|
YY1 antibody - ChIP Grade (ab38422) |
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Protein G purified
|
|
Storage buffer
|
Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.250 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this YY1 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for YY1 antibody - ChIP Grade images, references, products related to ab38422 and other tools.
|
| |
YY1 antibody - ChIP Grade images:
|
 Western blot - YY1 antibody (ab38422)
All lanes : YY1 antibody - ChIP Grade (ab38422) at 1/100 dilution
Lane 1 : HL60 cell lysate
Lane 2 : Mouse kidney tissue lysate
Predicted band size : 45 kDa
Observed band size : 45 kDa
Additional bands at : 30 kDa,65 kDa,75 kDa. We are unsure as to the identity of these extra bands.
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - YY1 antibody (ab38422)
Immunohistochemical analysis of YY1 expression in formalin-fixed and paraffin-embedded human hepatocarcinoma tissue using 1/50 ab38422.
|
| |
References for YY1 antibody - ChIP Grade (ab38422)
|
|
This product has been used in:
Song G & Wang L Nuclear receptor SHP activates miR-206 expression via a cascade dual inhibitory mechanism. PLoS One 4:e6880 (2009). ChIP. PubMed: 19721712
If you publish research using ab38422 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to YY1
|
| |
YY1 antibody - ChIP Grade - more information
|
Customer reviews (feedback) regarding YY1 antibody - ChIP Grade |
Customer FAQs regarding YY1 antibody - ChIP Grade |
Protocols for YY1 antibody - ChIP Grade |
Price and availability of products related to YY1 antibody - ChIP Grade |
| |
YY1 antibody - ChIP Grade - related products:
|
|
|
| |
YY1 antibody - ChIP Grade - other tools:
|
Contact Abcam with a Technical Enquiry about YY1 antibody - ChIP Grade |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
