Overview

  • Product nameAnti-YY1 antibody [EPR4651]
    See all YY1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4651] to YY1
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human YY1.

  • Positive control
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC.
  • Target

    • FunctionMultifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
    • Sequence similaritiesBelongs to the YY transcription factor family.
      Contains 4 C2H2-type zinc fingers.
    • Cellular localizationNucleus matrix. Associated with the nuclear matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CF1 antibody
      • Delta antibody
      • Delta transcription factor antibody
      • INO80 complex subunit S antibody
      • INO80S antibody
      • NF E1 antibody
      • NF-E1 antibody
      • NFE1 antibody
      • OTTHUMP00000197459 antibody
      • Transcriptional repressor protein YY1 antibody
      • TYY1_HUMAN antibody
      • UCR motif DNA binding protein antibody
      • UCRBP antibody
      • Yin and yang 1 antibody
      • Yin and Yang 1 protein antibody
      • Yin Yang 1 antibody
      • Ying Yang 1 antibody
      • YY 1 antibody
      • YY 1 transcription factor antibody
      • YY-1 antibody
      • YY1 antibody
      • YY1 transcription factor antibody
      see all

    Anti-YY1 antibody [EPR4651] images

    • All lanes : Anti-YY1 antibody [EPR4651] (ab109228) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : Y79 cell lysates
      Lane 3 : HuT-78 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 45 kDa
    • Immunohistochemical analysis of YY1 in paraffin embedded Human colon tissue, using a 1/250 dilution of ab109228.

    References for Anti-YY1 antibody [EPR4651] (ab109228)

    ab109228 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"