Recombinant
RabMAb

Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)

Overview

  • Product name
    Anti-YY1 antibody [EPR4652] - Nuclear Loading Control
    See all YY1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4652] to YY1 - Nuclear Loading Control
  • Tested applications
    Suitable for: Flow Cyt, WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human YY1 aa 250-350 (internal sequence).

  • Positive control
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-YY1 antibody (Alexa Fluor® 488) [EPR4652] - Nuclear Loading Control (ab199814)

    Anti-YY1 antibody (Alexa Fluor® 647) [EPR4652] - Nuclear Loading Control (ab199815)

    Anti-YY1 antibody (HRP) [EPR4652] - Nuclear Loading Control (ab199998)

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab109237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/2000 - 1/10000. Predicted molecular weight: 45 kDa.
IP 1/30.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/250 - 1/500.

ICC/IF 1/50.

For unpurified use at 1/100 - 1/250.

Target

  • Function
    Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
  • Sequence similarities
    Belongs to the YY transcription factor family.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus matrix. Associated with the nuclear matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CF1 antibody
    • Delta antibody
    • Delta transcription factor antibody
    • INO80 complex subunit S antibody
    • INO80S antibody
    • NF E1 antibody
    • NF-E1 antibody
    • NFE1 antibody
    • OTTHUMP00000197459 antibody
    • Transcriptional repressor protein YY1 antibody
    • TYY1_HUMAN antibody
    • UCR motif DNA binding protein antibody
    • UCRBP antibody
    • Yin and yang 1 antibody
    • Yin and Yang 1 protein antibody
    • Yin Yang 1 antibody
    • Ying Yang 1 antibody
    • YY 1 antibody
    • YY 1 transcription factor antibody
    • YY-1 antibody
    • YY1 antibody
    • YY1 transcription factor antibody
    see all

Anti-YY1 antibody [EPR4652] - Nuclear Loading Control images

  • ab109237 staining YY1 in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/30. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/10000 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 45 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling YY1 with purified ab109237 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling YY1 with unpurified ab109237 at 1/100.

  • All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/50000 dilution (purified)

    Lane 1 : Y79 cell lysate
    Lane 2 : HuT-78 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 45 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human tonsil tissue labelling YY1 with unpurified ab109237 at 1/250.

  • All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/2000 dilution (purified)

    Lane 1 : Mouse heart
    Lane 2 : Rat heart

    Lysates/proteins at 10 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 45 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labelling YY1 with unpurified ab109237 at 1/250.

  • Immunocytochemistry/Immunofluorescence analysis of HUT-78 cells labelling YY1 with purified ab109237 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

  • All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/1000 dilution (unpurified)

    Lane 1 : Daudi cell lysate
    Lane 2 : Y-79 cell lysate
    Lane 3 : HuT-78 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 45 kDa
  • ab109237 (purified) at 1/30 immunoprecipitating YY1 in Y79 cells. For western blotting, a Peroxidase-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

References for Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)

This product has been referenced in:
  • Kim JS  et al. Diagnostic and prognostic relevance of CP2c and YY1 expression in hepatocellular carcinoma. Oncotarget 8:24389-24400 (2017). IHC-P ; Human . Read more (PubMed: 28412749) »
  • Yamamoto H  et al. Amla Enhances Mitochondrial Spare Respiratory Capacity by Increasing Mitochondrial Biogenesis and Antioxidant Systems in a Murine Skeletal Muscle Cell Line. Oxid Med Cell Longev 2016:1735841 (2016). WB . Read more (PubMed: 27340504) »

See all 3 Publications for this product

Product Wall

Application
Western blot
Sample
Astatotilapia burtoni Tissue lysate - nuclear (Brain nuclear extracts)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
25 µg
Specification
Brain nuclear extracts
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Sebastian Alvarado

Verified customer

Submitted May 04 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat cell lysate)
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Loading amount
80 µg
Specification
Jurkat cell lysate
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Aug 03 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Sample
Human Tissue sections (human colon)
Specification
human colon
Permeabilization
No
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted Dec 10 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Sample
Human Tissue sections (kidney)
Specification
kidney
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (murine erythroleukemia (MEL cell))
Loading amount
30 µg
Specification
murine erythroleukemia (MEL cell)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Min Young Kim

Verified customer

Submitted Jan 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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