Anti-YY1 antibody - Nuclear Loading Control (ab43058)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab43058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 45 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionMultifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
  • Sequence similaritiesBelongs to the YY transcription factor family.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localizationNucleus matrix. Associated with the nuclear matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CF1 antibody
    • Delta antibody
    • Delta transcription factor antibody
    • INO80 complex subunit S antibody
    • INO80S antibody
    • NF E1 antibody
    • NF-E1 antibody
    • NFE1 antibody
    • OTTHUMP00000197459 antibody
    • Transcriptional repressor protein YY1 antibody
    • TYY1_HUMAN antibody
    • UCR motif DNA binding protein antibody
    • UCRBP antibody
    • Yin and yang 1 antibody
    • Yin and Yang 1 protein antibody
    • Yin Yang 1 antibody
    • Ying Yang 1 antibody
    • YY 1 antibody
    • YY 1 transcription factor antibody
    • YY-1 antibody
    • YY1 antibody
    • YY1 transcription factor antibody
    see all

Anti-YY1 antibody - Nuclear Loading Control images

  • Anti-YY1 antibody - Nuclear Loading Control (ab43058) at 2 µg/ml + Jurkat nuclear extract lysate (ab14844) at 20 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab43058 stained human HEK 293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab43058, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HepG2 and MCF7 cells.

  • IHC image of YY1 staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab43058, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-YY1 antibody - Nuclear Loading Control (ab43058)

This product has been referenced in:
  • Bengani H  et al. Identification and Validation of a Putative Polycomb Responsive Element in the Human Genome. PLoS One 8:e67217 (2013). Read more (PubMed: 23805300) »

See 1 Publication for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Specification NIH-3T3
Sample Mouse Cell lysate - whole cell (NIH-3T3)
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Oct 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"