Anti-YY1 associated factor 2 antibody (ab55976)

Overview

  • Product name
    Anti-YY1 associated factor 2 antibody
    See all YY1 associated factor 2 primary antibodies
  • Description
    Rabbit polyclonal to YY1 associated factor 2
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: QSKKEKKDKV EKEKSEKETT SKKNSHKKTR PRLKNVDRSS AQHLEVTVGD, corresponding to internal sequence amino acids 73-122 of Human YY1 associated factor 2

  • Positive control
    • WB: Jurkat cell lysate. IHC-P: Human muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab55976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 20 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Images

  • Anti-YY1 associated factor 2 antibody (ab55976) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling YY1 associated factor 2 with ab55976 at 4-8µg/ml. Arrow indicate positively labelled skeletal muscle cells. Magnification: 400X.

References

ab55976 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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