Recombinant fragment: AQAPADPGRE GHLEQRILQV LTEAGSPVKL AQLVKECQAP KRELNQVLYR MKKELKVSLT SPATWCLGGT DPEGEGPAEL ALSSPAKRPQ QHAATIPET, corresponding to N terminal amino acids 2-100 of Human Z DNA binding protein (NP_110403) with a proprietary tag.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 46 kDa.
Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Place sample in 1X citrate buffer (pH 6.0) in pressure cooker under 125oC for 4min and under 90oC for 45min.
FunctionMay play a role in host defense against tumors and pathogens. Binds Z-DNA.
Tissue specificityHighly expressed in lymphatic tissues including lymph node, leukocytes, tonsil, bone marrow and spleen. Expressed to a lesser extent in thymus, lung and liver.