Overview

  • Product name
  • Description
    Rabbit polyclonal to ZADH1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: ILDGNSLEKV DPQLVDGHLS YFLGAIGMPG LTSLIGIQEK GHITAGSNKT , corresponding to a region within internal sequence amino acids 108 - 157 of Human ZADH1 (NP_689657)

  • Positive control
    • MCF7 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the NADP-dependent oxidoreductase L4BD family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 15 oxoprostaglandin 13 reductase antibody
    • 15 oxoprostaglandin delta 13 reductase antibody
    • 15-oxoprostaglandin 13-reductase antibody
    • DKFZp686P10120 antibody
    • FLJ39091 antibody
    • FLJ99229 antibody
    • PGR 2 antibody
    • PGR2 antibody
    • PRG-2 antibody
    • Prostaglandin reductase 2 antibody
    • PTGR 2 antibody
    • PTGR2 antibody
    • PTGR2_HUMAN antibody
    • Zinc binding alcohol dehydrogenase domain containing 1 antibody
    • Zinc binding alcohol dehydrogenase domain containing protein 1 antibody
    • Zinc-binding alcohol dehydrogenase domain-containing protein 1 antibody
    see all

Images

  • Anti-ZADH1 antibody (ab84711) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Gedik N  et al. Proteomics/phosphoproteomics of left ventricular biopsies from patients with surgical coronary revascularization and pigs with coronary occlusion/reperfusion: remote ischemic preconditioning. Sci Rep 7:7629 (2017). Read more (PubMed: 28794502) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab84711.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up