Overview

  • Product name
  • Description
    Rabbit polyclonal to ZAK
  • Specificity
    ab65249 detects endogenous levels of total ZAK protein.
  • Tested applications
    Suitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from C terminus of human ZAK

  • Positive control
    • HeLa cell extracts and HepG2 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).

Target

  • Function
    Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'.
  • Tissue specificity
    Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to the nucleus upon ultraviolet B irradiation.
  • Information by UniProt
  • Database links
  • Alternative names
    • AZK antibody
    • Cervical cancer suppressor gene 4 protein antibody
    • HCCS 4 antibody
    • HCCS-4 antibody
    • HCCS4 antibody
    • Human cervical cancer suppressor gene 4 protein antibody
    • Leucine zipper and sterile alpha motif containing kinase antibody
    • Leucine zipper- and sterile alpha motif-containing kinase antibody
    • Mitogen activated protein kinase kinase kinase MLT antibody
    • Mitogen-activated protein kinase kinase kinase MLT antibody
    • Mixed lineage kinase 7 antibody
    • Mixed lineage kinase related kinase antibody
    • Mixed lineage kinase related kinase MRK beta antibody
    • Mixed lineage kinase with a leucine zipper and a sterile alpha motif antibody
    • Mixed lineage kinase-related kinase antibody
    • MLK 7 antibody
    • MLK like mitogen activated protein triple kinase antibody
    • MLK related kinase antibody
    • MLK-like mitogen-activated protein triple kinase antibody
    • MLK-related kinase antibody
    • MLK7 antibody
    • Mlklak antibody
    • MLT antibody
    • MLTK_HUMAN antibody
    • MRK antibody
    • Sterile alpha motif and leucine zipper containing kinase AZK antibody
    • Sterile alpha motif- and leucine zipper-containing kinase AZK antibody
    • Zak antibody
    see all

Images

  • ab65249 at 1/500 dilution staining ZAK in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • All lanes : Anti-ZAK antibody (ab65249) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 91 kDa
    Observed band size : 91 kDa

References

ab65249 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry. The cellular location information was taken from SwissProt database. Please see the link below (Comments section). http://br.expasy.org/cgi-bin/niceprot.pl?Q9NYL2 I hope this information will be helpful. If there...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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