• Product nameAnti-ZAK antibody
    See all ZAK primary antibodies
  • Description
    Rabbit polyclonal to ZAK
  • Specificityab65249 detects endogenous levels of total ZAK protein.
  • Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from C terminus of human ZAK

  • Positive control
    • HeLa cell extracts and HepG2 cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab65249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).


  • FunctionStress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'.
  • Tissue specificityUbiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localizationCytoplasm. Nucleus. Translocates to the nucleus upon ultraviolet B irradiation.
  • Information by UniProt
  • Database links
  • Alternative names
    • AZK antibody
    • Cervical cancer suppressor gene 4 protein antibody
    • HCCS 4 antibody
    • HCCS-4 antibody
    • HCCS4 antibody
    • Human cervical cancer suppressor gene 4 protein antibody
    • Leucine zipper and sterile alpha motif containing kinase antibody
    • Leucine zipper- and sterile alpha motif-containing kinase antibody
    • Mitogen activated protein kinase kinase kinase MLT antibody
    • Mitogen-activated protein kinase kinase kinase MLT antibody
    • Mixed lineage kinase 7 antibody
    • Mixed lineage kinase related kinase antibody
    • Mixed lineage kinase related kinase MRK beta antibody
    • Mixed lineage kinase with a leucine zipper and a sterile alpha motif antibody
    • Mixed lineage kinase-related kinase antibody
    • MLK 7 antibody
    • MLK like mitogen activated protein triple kinase antibody
    • MLK related kinase antibody
    • MLK-like mitogen-activated protein triple kinase antibody
    • MLK-related kinase antibody
    • MLK7 antibody
    • Mlklak antibody
    • MLT antibody
    • MLTK_HUMAN antibody
    • MRK antibody
    • Sterile alpha motif and leucine zipper containing kinase AZK antibody
    • Sterile alpha motif- and leucine zipper-containing kinase AZK antibody
    • Zak antibody
    see all

Anti-ZAK antibody images

  • ab65249 at 1/500 dilution staining ZAK in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • All lanes : Anti-ZAK antibody (ab65249) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 91 kDa
    Observed band size : 91 kDa

References for Anti-ZAK antibody (ab65249)

ab65249 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The cellular location information was taken from SwissProt database. Please see the link below (Comments section). http://br.expasy.org/cgi-bin/niceprot.pl?Q9NYL2 I hope this information will be helpful. If there...

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