Overview

  • Product name
    Anti-ZAK antibody - N-terminal
    See all ZAK primary antibodies
  • Description
    Rabbit polyclonal to ZAK - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to a region within N terminal amino acids 1-259 of Human ZAK.

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, Molt-4 and Raji cell lysates; A431 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 91 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'.
  • Tissue specificity
    Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to the nucleus upon ultraviolet B irradiation.
  • Information by UniProt
  • Database links
  • Alternative names
    • AZK antibody
    • Cervical cancer suppressor gene 4 protein antibody
    • HCCS 4 antibody
    • HCCS-4 antibody
    • HCCS4 antibody
    • Human cervical cancer suppressor gene 4 protein antibody
    • Leucine zipper and sterile alpha motif containing kinase antibody
    • Leucine zipper- and sterile alpha motif-containing kinase antibody
    • Mitogen activated protein kinase kinase kinase MLT antibody
    • Mitogen-activated protein kinase kinase kinase MLT antibody
    • Mixed lineage kinase 7 antibody
    • Mixed lineage kinase related kinase antibody
    • Mixed lineage kinase related kinase MRK beta antibody
    • Mixed lineage kinase with a leucine zipper and a sterile alpha motif antibody
    • Mixed lineage kinase-related kinase antibody
    • MLK 7 antibody
    • MLK like mitogen activated protein triple kinase antibody
    • MLK related kinase antibody
    • MLK-like mitogen-activated protein triple kinase antibody
    • MLK-related kinase antibody
    • MLK7 antibody
    • Mlklak antibody
    • MLT antibody
    • MLTK_HUMAN antibody
    • MRK antibody
    • Sterile alpha motif and leucine zipper containing kinase AZK antibody
    • Sterile alpha motif- and leucine zipper-containing kinase AZK antibody
    • Zak antibody
    see all

Images

  • Anti-ZAK antibody - N-terminal (ab154602) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg

    Predicted band size: 91 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling ZAK with ab154602 at 1/200 dilution. Lower panel is merged with DNA probe.

References

ab154602 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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