The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 306 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Use at an assay dependent dilution.
Use a concentration of 10 µg/ml.
FunctionBinds in a species-specific manner to the zona pellucida of the egg. May be involved in gamete recognition and/or signaling.
Tissue specificityIn testis, primarily in haploid spermatids.
DomainThe MAM domains probably mediate sperm adhesion to the zona pellucida. During sperm migration through the reproductive tracts, the mucin-like domain might inhibit inappropriate trapping of spermatozoa or promoting adhesion to the oviductal isthmus. The VWFD domain 2 may mediate covalent oligomerization.
Cellular localizationCell membrane. Exclusively on the apical region of the sperm head.