Overview

  • Product nameAnti-ZAN antibody
    See all ZAN primary antibodies
  • Description
    Mouse monoclonal to ZAN
  • Tested applicationsSuitable for: WB, ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 19-114 of Human ZAN with a proprietary tag (NP_003377).

  • Positive control
    • Recombinant Human ZAN Protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 306 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use a concentration of 10 µg/ml.

Target

  • FunctionBinds in a species-specific manner to the zona pellucida of the egg. May be involved in gamete recognition and/or signaling.
  • Tissue specificityIn testis, primarily in haploid spermatids.
  • Sequence similaritiesContains 1 EGF-like domain.
    Contains 3 MAM domains.
    Contains 4 TIL (trypsin inhibitory-like) domains.
    Contains 5 VWFC domains.
    Contains 4 VWFD domains.
  • DomainThe MAM domains probably mediate sperm adhesion to the zona pellucida.
    During sperm migration through the reproductive tracts, the mucin-like domain might inhibit inappropriate trapping of spermatozoa or promoting adhesion to the oviductal isthmus.
    The VWFD domain 2 may mediate covalent oligomerization.
  • Cellular localizationCell membrane. Exclusively on the apical region of the sperm head.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000211358 antibody
    • OTTHUMP00000211359 antibody
    • OTTHUMP00000211360 antibody
    • OTTHUMP00000211361 antibody
    • OTTHUMP00000211362 antibody
    • OTTHUMP00000211363 antibody
    • ZAN antibody
    • ZAN_HUMAN antibody
    • Zonadhesin antibody
    see all

Anti-ZAN antibody images

  • Anti-ZAN antibody (ab118116) at 5 µg/ml + recombinant immunogen at 0.2 µg
    Developed using the ECL technique

    Predicted band size : 306 kDa
  • ab118116 at 10 µg/ml as a capture antibody. The detection limit for recombinant tagged ZAN is 0.03 ng/ml.

References for Anti-ZAN antibody (ab118116)

ab118116 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"