The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionPlays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Tissue specificityExpressed in T- and natural killer cells.
Involvement in diseaseDefects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. Contains 1 protein kinase domain. Contains 2 SH2 domains.
DomainThe SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
Post-translational modificationsPhosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
Cellular localizationCytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
Zeta chain associated protein kinase 70kD antibody
Zeta chain associated protein kinase 70kDa antibody
Zeta chain associated protein kinase 70kDa isoform 1 antibody
Zeta chain associated protein kinase 70kDa isoform 2 antibody
Zeta chain TCR associated protein kinase 70kD antibody
Zeta chain TCR associated protein kinase 70kDa antibody
References for Anti-ZAP70 antibody [1E7.2] (FITC) (ab119853)
This product has been referenced in:
Preobrazhensky SN & Bahler DW Optimization of flow cytometric measurement of ZAP-70 in chronic lymphocytic leukemia. Cytometry B Clin Cytom74:118-27 (2008).
Read more (PubMed: 17948236) »
Letestu R et al. Evaluation of ZAP-70 expression by flow cytometry in chronic lymphocytic leukemia: A multicentric international harmonization process. Cytometry B Clin Cytom70:309-14 (2006).
Read more (PubMed: 16906588) »