• Product nameAnti-ZAP70 (phospho Y292) antibody
    See all ZAP70 primary antibodies
  • Description
    Rabbit polyclonal to ZAP70 (phospho Y292)
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from a region of human ZAP70 that contains tyrosine 292 (unfortunately, the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • Jurkat cells treated with hydrogen peroxide.
  • General notesCentrifuge before opening to settle contents.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: Dulbecco's PBS without magnesium and calcium, 50% Glycerol, PBS, 1mg/ml BSA, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated ZAP70. The final product is generated by affinity chromatography using a ZAP70-derived peptide that is phosphorylated at threonine 292.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab12868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 74.3 kDa.


  • FunctionPlays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
  • Tissue specificityExpressed in T- and natural killer cells.
  • Involvement in diseaseDefects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
    Contains 1 protein kinase domain.
    Contains 2 SH2 domains.
  • DomainThe SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
  • Post-translational
    Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
  • Cellular localizationCytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70 kDa zeta associated protein antibody
    • 70 kDa zeta-associated protein antibody
    • EC antibody
    • FLJ17670 antibody
    • FLJ17679 antibody
    • Selective T cell defect antibody
    • SRK antibody
    • STD antibody
    • Syk related tyrosine kinase antibody
    • Syk-related tyrosine kinase antibody
    • Truncated ZAP kinase antibody
    • Tyrosine protein kinase ZAP70 antibody
    • Tyrosine-protein kinase ZAP-70 antibody
    • TZK antibody
    • ZAP 70 antibody
    • ZAP70 antibody
    • ZAP70_HUMAN antibody
    • Zeta chain associated protein kinase 70kD antibody
    • Zeta chain associated protein kinase 70kDa antibody
    • Zeta chain associated protein kinase 70kDa isoform 1 antibody
    • Zeta chain associated protein kinase 70kDa isoform 2 antibody
    • Zeta chain TCR associated protein kinase 70kD antibody
    • Zeta chain TCR associated protein kinase 70kDa antibody
    see all

Anti-ZAP70 (phospho Y292) antibody images

  • Predicted band size : 74.3 kDa

    Peptide Competition and Phosphatase Treatment: Lysates prepared from Jurkat cells left untreated (1) or treated with hydrogen peroxide (2-6) were either left untreated (1-5) or treated with lambda phosphatase (6). Lysates were then incubated with ab12868 following prior incubation with: no peptide (1, 6), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphotyrosine-containing peptide (4) or, the phosphopeptide immunogen (5). The data show that only the peptide corresponding to ZAP70 [phospho Y292] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

References for Anti-ZAP70 (phospho Y292) antibody (ab12868)

This product has been referenced in:
  • Haas A  et al. Intracellular Phospho-Flow cytometry reveals novel insights into TCR proximal signaling events. A comparison with Western blot. Cytometry A 73:799-807 (2008). WB ; Human . Read more (PubMed: 18548611) »

See 1 Publication for this product

Product Wall

Thank you again for your patience, I have just heard back from the originator of this antibody. Are you not seeing any signal at all? The originator said that it worked well for them on an endogenous system. Also, ZAP-70 tyrosine 292 is the negativ...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab12868. You mentioned that you are using Jurkat cell lysates; did you treat the lysates with hydrogen peroxide? Jurkat cells treated with hydrogen peroxide are ...

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