Anti-ZAP70 (phospho Y319) antibody (ab194792)

Overview

  • Product name
    Anti-ZAP70 (phospho Y319) antibody
    See all ZAP70 primary antibodies
  • Description
    Rabbit polyclonal to ZAP70 (phospho Y319)
  • Specificity
    ab194792 could cross react with human SYK
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human ZAP70 (phospho Y319).
    Database link: P43404

  • Positive control
    • Jurkat cell extracts. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab194792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 70 kDa.
ICC/IF 1/50 - 1/100.

Target

  • Cellular localization
    Cytoplasm. Cell membrane; Peripheral membrane protein. Note=In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity)
  • Database links
  • Alternative names
    • 70 KDa Zeta-Associated Protein2 antibody
    • 70 KDa Zeta-Chain Associated Protein antibody
    • EC 2.7.10 antibody
    • EC 2.7.10.2 antibody
    • SRK antibody
    • STCD antibody
    • STD antibody
    • Syk-Related Tyrosine Kinase antibody
    • Tyrosine-Protein Kinase ZAP-70 antibody
    • TZK antibody
    • ZAP-70 antibody
    • Zeta-Chain (TCR) Associated ProteinKinase (70 KD) antibody
    • Zeta-Chain Associated Protein Kinase, 70kD antibody
    see all

Images

  • All lanes : Anti-ZAP70 (phospho Y319) antibody (ab194792) at 1/500 dilution

    Lane 1 : Jurkat cell extract with peptide
    Lane 2 : Jurkat cell extract


    Predicted band size : 70 kDa
  • Immunofluorescent staining of methanol fixed HeLa cells, labeling ZAP70 with ab194792 at 1/50 dilution.

References

ab194792 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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