Overview

  • Product nameAnti-ZAP70 (phospho Y492) antibody [EP2291Y]
    See all ZAP70 primary antibodies
  • Description
    Rabbit monoclonal [EP2291Y] to ZAP70 (phospho Y492)
  • Specificityab76306 only detects ZAP70 phosphorylated on Tyrosine 492.
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Tyr492 of human ZAP70

  • Positive control
    • Jurkat cell lysates, untreated or treated with pervanadate.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IP 1/30.
  • Application notesIs unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • FunctionPlays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
    • Tissue specificityExpressed in T- and natural killer cells.
    • Involvement in diseaseDefects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
      Contains 1 protein kinase domain.
      Contains 2 SH2 domains.
    • DomainThe SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
    • Cellular localizationCytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
    • Information by UniProt
    • Database links
    • Alternative names
      • 70 kDa zeta associated protein antibody
      • 70 kDa zeta-associated protein antibody
      • EC 2.7.10.2 antibody
      • FLJ17670 antibody
      • FLJ17679 antibody
      • Selective T cell defect antibody
      • SRK antibody
      • STD antibody
      • Syk related tyrosine kinase antibody
      • Syk-related tyrosine kinase antibody
      • Truncated ZAP kinase antibody
      • Tyrosine protein kinase ZAP70 antibody
      • Tyrosine-protein kinase ZAP-70 antibody
      • TZK antibody
      • ZAP 70 antibody
      • ZAP70 antibody
      • ZAP70_HUMAN antibody
      • Zeta chain associated protein kinase 70kD antibody
      • Zeta chain associated protein kinase 70kDa antibody
      • Zeta chain associated protein kinase 70kDa isoform 1 antibody
      • Zeta chain associated protein kinase 70kDa isoform 2 antibody
      • Zeta chain TCR associated protein kinase 70kD antibody
      • Zeta chain TCR associated protein kinase 70kDa antibody
      see all

    Anti-ZAP70 (phospho Y492) antibody [EP2291Y] images

    • All lanes : Anti-ZAP70 (phospho Y492) antibody [EP2291Y] (ab76306) at 1/20000 dilution

      Lane 1 : Jurkat cell lysates, untreated
      Lane 2 : Jurkat cell lysates, treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 70 kDa
      Observed band size : 70 kDa

    References for Anti-ZAP70 (phospho Y492) antibody [EP2291Y] (ab76306)

    This product has been referenced in:
    • Chiang YJ  et al. CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-Cell adhesion and migration. PLoS One 8:e61761 (2013). WB . Read more (PubMed: 23620790) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"