Overview

  • Product name
    Anti-ZBED4 antibody
  • Description
    Rabbit polyclonal to ZBED4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 33-171 of Human ZBED4.

  • Positive control
    • Human testis tissue; Human U-2 OS cells; RT-4 and U-251 MG cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab150927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-10.
WB 1/250 - 1/500. Predicted molecular weight: 130 kDa.

Target

Images

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ZBED4 with ab150927 at 1/20 dilution.
  • All lanes : Anti-ZBED4 antibody (ab150927) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Predicted band size: 130 kDa

  • Immunofluorescent analysis of PFA-fixed, Triton X-100-permeabilized Human U-2 OS cells labeling ZBED4 with ab150927 at 4 µg/ml.

References

ab150927 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab150927.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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