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Rabbit polyclonal to ZBT10
Predicted to work with:
Rabbit, Cow, Pig, Chimpanzee, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 50-100 of Human ZBT10 (NP_076418.3).
Jurkat and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7-8
Concentration information loading...
Immunogen affinity purified
ab117786 was affinity purified using an epitope specific to ZBT10 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
Use at 2-10 µg/mg of lysate.
May be involved in transcriptional regulation.
Contains 1 BTB (POZ) domain.
Contains 2 C2H2-type zinc fingers.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Western blot - Anti-ZBT10 antibody (ab117786)
All lanes : Anti-ZBT10 antibody (ab117786) at 0.1 µg/ml Lane 1 : 293T whole cell lysates at 50 µg Lane 2 : 293T whole cell lysates at 15 µg Lane 3 : Jurkat whole cell lysates at 50 µg Developed using the ECL technique Predicted band size : 95 kDa Exposure time : 30 seconds
Immunoprecipitation - Anti-ZBT10 antibody (ab117786)
Detection of ZBT10 by Western Blot of Immunprecipitate.
ab117786 at 1µg/ml staining ZBT10 in 293T whole cell lysate immunoprecipitated using ab117786 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"