Overview

  • Product name
  • Description
    Rabbit polyclonal to ZBT10
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 797-847 of Human ZBT10 (NP_076418.3).

  • Positive control
    • Jurkat and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab117789 was affinity purified using an epitope specific to ZBT10 immobilized on solid support .
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    May be involved in transcriptional regulation.
  • Sequence similarities
    Contains 1 BTB (POZ) domain.
    Contains 2 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ12752 antibody
    • OTTHUMP00000207119 antibody
    • RINZF antibody
    • RINZFC antibody
    • ZBT10_HUMAN antibody
    • ZBTB10 antibody
    • Zinc finger and BTB domain containing protein 10 antibody
    • Zinc finger and BTB domain-containing protein 10 antibody
    • Zinc finger protein RIN ZF antibody
    see all

Images

  • All lanes : Anti-ZBT10 antibody (ab117789) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysates at 50 µg
    Lane 2 : 293T whole cell lysates at 15 µg
    Lane 3 : Jurkat whole cell lysates at 50 µg

    Developed using the ECL technique

    Predicted band size : 95 kDa


    Exposure time : 30 seconds
  • Detection of ZBT10 by Western Blot of Immunprecipitate.
    ab117789 at 1µg/ml staining ZBT10 in 293T whole cell lysate immunoprecipitated using ab117789 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

References

ab117789 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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