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Overview

  • Product name
    Anti-ZBT10 antibody
    See all ZBT10 primary antibodies
  • Description
    Rabbit polyclonal to ZBT10
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 797-847 of Human ZBT10 (NP_076418.3).

  • Positive control
    • Jurkat and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab117789 was affinity purified using an epitope specific to ZBT10 immobilized on solid support .
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    May be involved in transcriptional regulation.
  • Sequence similarities
    Contains 1 BTB (POZ) domain.
    Contains 2 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • FLJ12752 antibody
      • OTTHUMP00000207119 antibody
      • RINZF antibody
      • RINZFC antibody
      • ZBT10_HUMAN antibody
      • ZBTB10 antibody
      • Zinc finger and BTB domain containing protein 10 antibody
      • Zinc finger and BTB domain-containing protein 10 antibody
      • Zinc finger protein RIN ZF antibody
      see all

    Anti-ZBT10 antibody images

    • Western blot - Anti-ZBT10 antibody (ab117789)
      Western blot - Anti-ZBT10 antibody (ab117789)
      All lanes : Anti-ZBT10 antibody (ab117789) at 0.1 µg/ml

      Lane 1 : 293T whole cell lysates at 50 µg
      Lane 2 : 293T whole cell lysates at 15 µg
      Lane 3 : Jurkat whole cell lysates at 50 µg

      Developed using the ECL technique

      Predicted band size : 95 kDa


      Exposure time : 30 seconds
    • Immunoprecipitation - Anti-ZBT10 antibody (ab117789)
      Immunoprecipitation - Anti-ZBT10 antibody (ab117789)
      Detection of ZBT10 by Western Blot of Immunprecipitate.
      ab117789 at 1µg/ml staining ZBT10 in 293T whole cell lysate immunoprecipitated using ab117789 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 seconds.

    References for Anti-ZBT10 antibody (ab117789)

    ab117789 has not yet been referenced specifically in any publications.

    Publishing research using ab117789? Please let us know so that we can cite the reference in this datasheet.

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