Overview

  • Product name
  • Description
    Rabbit polyclonal to ZBT10
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human ZBT10.

    (Peptide available as ab90846.)

  • Positive control
    • This antibody gave a positive signal in human liver tissue lysate and the following whole cell lysates: HeLa; HEK293; HepG2; HUVEC; SHSY-5Y.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).

Target

  • Function
    May be involved in transcriptional regulation.
  • Sequence similarities
    Contains 1 BTB (POZ) domain.
    Contains 2 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ12752 antibody
    • OTTHUMP00000207119 antibody
    • RINZF antibody
    • RINZFC antibody
    • ZBT10_HUMAN antibody
    • ZBTB10 antibody
    • Zinc finger and BTB domain containing protein 10 antibody
    • Zinc finger and BTB domain-containing protein 10 antibody
    • Zinc finger protein RIN ZF antibody
    see all

Images

  • All lanes : Anti-ZBT10 antibody (ab80879) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
    Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 92 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 46 kDa,67 kDa (possible isoform). We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References

ab80879 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab80879.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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