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Rabbit polyclonal to ZBT10
Predicted to work with:
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human ZBT10.
(Peptide available as
This antibody gave a positive signal in human liver tissue lysate and the following whole cell lysates: HeLa; HEK293; HepG2; HUVEC; SHSY-5Y.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).
May be involved in transcriptional regulation.
Contains 1 BTB (POZ) domain.
Contains 2 C2H2-type zinc fingers.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Western blot - ZBT10 antibody (ab80879)
All lanes :
Anti-ZBT10 antibody (ab80879) at 1 µg/ml
Lane 1 :
Human liver tissue lysate - total protein (
Lane 2 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 :
HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 :
HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 :
HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 6 :
SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
100 kDa (
why is the actual band size different from the predicted?
Additional bands at:
46 kDa, 67 kDa (possible isoform). We are unsure as to the identity of these extra bands.
ab80879 detects a strong band at 100 kDa which is thought to be the target protein as well as a band at 67 kDa. It is thought that the bands around 67 kDa corresponds to isoform 2 which has a predicted molecular weight of 65 kDa (SwissProt data).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"