Anti-ZBTB2 antibody (ab117756)
Key features and details
- Rabbit polyclonal to ZBTB2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ZBTB2 antibody -
Description
Rabbit polyclonal to ZBTB2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 464-514 of Human ZBTB2 (NP_065912.1).
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Positive control
- 293T, Jurkat, HeLa and NIH3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab117756 was affinity purified using an epitope specific to ZBTB2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 57 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 57 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
May be involved in transcriptional regulation. -
Sequence similarities
Contains 1 BTB (POZ) domain.
Contains 4 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 484036 Dog
- Entrez Gene: 100060294 Horse
- Entrez Gene: 57621 Human
- Entrez Gene: 381990 Mouse
- Entrez Gene: 308126 Rat
- Entrez Gene: 704782 Rhesus monkey
- SwissProt: Q8N680 Human
- Unigene: 520073 Human
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Alternative names
- bA351K16.2 antibody
- Gm1103 antibody
- KIAA1483 antibody
see all
Images
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All lanes : Anti-ZBTB2 antibody (ab117756) at 0.04 µg/ml
Lane 1 : 293T cell line lysate at 50 µg
Lane 2 : 293T cell line lysate at 15 µg
Lane 3 : Jurkat cell line lysate at 50 µg
Lane 4 : HeLa cell line lysate at 50 µg
Lane 5 : NIH3T3 cell line lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 30 seconds -
Detection of ZBTB2 by Western Blot of Immunprecipitate.
ab117756 at 1µg/ml staining ZBTB2 in 293T whole cell lysate immunoprecipitated using ab117756 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab117756 has not yet been referenced specifically in any publications.