Overview

  • Product name
  • Description
    Rabbit polyclonal to ZBTB2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 464-514 of Human ZBTB2 (NP_065912.1).

  • Positive control
    • 293T, Jurkat, HeLa and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab117756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 57 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-ZBTB2 antibody (ab117756) at 0.04 µg/ml

    Lane 1 : 293T cell line lysate at 50 µg
    Lane 2 : 293T cell line lysate at 15 µg
    Lane 3 : Jurkat cell line lysate at 50 µg
    Lane 4 : HeLa cell line lysate at 50 µg
    Lane 5 : NIH3T3 cell line lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 57 kDa


    Exposure time : 30 seconds
  • Detection of ZBTB2 by Western Blot of Immunprecipitate.
    ab117756 at 1µg/ml staining ZBTB2 in 293T whole cell lysate immunoprecipitated using ab117756 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab117756 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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