Overview

  • Product name
  • Description
    Rabbit polyclonal to ZBTB38
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 150-200 of Human ZBTB38 (NP_001073881.2 (GeneID 253461)).

  • Positive control
    • HeLa whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.50
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 134 kDa).
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Acts as a transcriptional activator. May be involved in the differentiation and/or survival of late postmitotic neurons.
  • Sequence similarities
    Contains 1 BTB (POZ) domain.
    Contains 10 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIBZ antibody
    • FLJ22332 antibody
    • FLJ31131 antibody
    • FLJ35036 antibody
    • ZBT38_HUMAN antibody
    • ZBTB38 antibody
    • Ziinc finger and BTB domain containing protein 38 antibody
    • Zinc finger and BTB domain-containing protein 38 antibody
    see all

Images

  • All lanes : Anti-ZBTB38 antibody (ab112051) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 134 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  •  

     

  • Lane 1 : Anti-ZBTB38 antibody (ab112051) at 1 µg/ml (lysate) 1 mg lysate/IP; 20% of IP loaded/lane)
    Lane 2 : Recombinant Human Neurotrophin 4 protein (denatured) (ab113151) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (6 µg ab112051/mg
    Lane 2 : Control IgG


    Predicted band size : 134 kDa

References

ab112051 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your call today and for your questions about ab112051.

Sincethis antibody has not been tested inChIP-Seq, I can offer a discount off a future purchase if you buy ab112051 now, test it in ChIP-Seqand submit feedback to us in the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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