Overview

  • Product nameAnti-ZBTB38 antibody
    See all ZBTB38 primary antibodies
  • Description
    Rabbit polyclonal to ZBTB38
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Pig
  • Immunogen

    A region within synthetic peptide: MSLSRDLKDD FHSDTVLSIL NEQRIRGILC DVTIIVEDTK FKAHSNVLAA, corresponding to N terminal amino acids 1-50 of Human ZBTB38

  • Positive control
    • HepG2 cell lysate and human kidney tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Peptide ELISA: 1/312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 135 kDa).

Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Anti-ZBTB38 antibody images

  • Anti-ZBTB38 antibody (ab50664) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG (1:100,000)

    Predicted band size : 135 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
  • Ab50664 (4-8ug/ml) staining human ZBTB38 in human kidney tissue by immunohistochemistry using paraffin embedded tissue. Epithelial cells of renal tubule (indicated with arrows) positively labelled.Ab50664 (4-8ug/ml) staining human ZBTB38 in human kidney tissue by immunohistochemistry using paraffin embedded tissue. Epithelial cells of renal tubule (indicated with arrows) positively labelled.

References for Anti-ZBTB38 antibody (ab50664)

ab50664 has not yet been referenced specifically in any publications.

Product Wall

Thank you for forwrding your images. They will be very helpful for our investigation into the problem with this antibody.

Please keep me updated about the results after trying the replacement antibody, and let me know if there is anything els...

Read More

Thank you for contacting us and letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge replacement vial of ab50664 which should arrive tomorrow on the order ***. Please keep me updated about the re...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"