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Rabbit polyclonal to ZBTB40
Predicted to work with:
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between residues 1140 and 1190 of human ZBTB40 (NP_055685.3)
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab86330 was affinity purified using an epitope specific to ZBTB40 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Predicted molecular weight: 138 kDa.
Use at 2-5 µg/mg of lysate.
May be involved in transcriptional regulation.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 1 BTB (POZ) domain. Contains 12 C2H2-type zinc fingers.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB40 antibody (ab86330)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ZBTB40 with ab86330 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - ZBTB40 antibody (ab86330)
All lanes :
Anti-ZBTB40 antibody (ab86330) at 0.1 µg/ml
Lane 1 :
293T whole cell lysates at 50 µg
Lane 2 :
293T whole cell lysates at 15 µg
Lane 3 :
293T whole cell lysates at 5 µg
Developed using the ECL technique.
Predicted band size:
Observed band size:
170 kDa (
why is the actual band size different from the predicted?
Immunoprecipitation - ZBTB40 antibody (ab86330)
Detection of ZBTB40 by Western Blot of Immunprecipitate.
ab86330, at 1 µg/ml, staining ZBTB40 in HeLa whole cell lysate immunoprecipitated using ab86330 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 3 minutes.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"