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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab86330 was affinity purified using an epitope specific to ZBTB40 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Predicted molecular weight: 138 kDa.
Use at 2-5 µg/mg of lysate.
Function May be involved in transcriptional regulation.
Sequence similarities Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 1 BTB (POZ) domain. Contains 12 C2H2-type zinc fingers.
Post-translational modifications Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization Nucleus.
Information by UniProt
Anti-ZBTB40 antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZBTB40 antibody (ab86330)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ZBTB40 with ab86330 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - ZBTB40 antibody (ab86330)
All lanes : Anti-ZBTB40 antibody (ab86330) at 0.1 µg/ml Lane 1 : 293T whole cell lysates at 50 µg Lane 2 : 293T whole cell lysates at 15 µg Lane 3 : 293T whole cell lysates at 5 µg Developed using the ECL technique Predicted band size : 138 kDa Observed band size : 170 kDa ( why is the actual band size different from the predicted?) Exposure time : 3 minutes
Immunoprecipitation - ZBTB40 antibody (ab86330)
Detection of ZBTB40 by Western Blot of Immunprecipitate.
ab86330, at 1 µg/ml, staining ZBTB40 in HeLa whole cell lysate immunoprecipitated using ab86330 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 3 minutes.
References for Anti-ZBTB40 antibody (ab86330)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"