Overview

  • Product name
  • Description
    Rabbit polyclonal to ZBTB40
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 1140 and 1190 of human ZBTB40 (NP_055685.3)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab86330 was affinity purified using an epitope specific to ZBTB40 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 138 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    May be involved in transcriptional regulation.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 BTB (POZ) domain.
    Contains 12 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BC059177 antibody
    • C230087D24 antibody
    • Gm571 antibody
    • KIAA0478 antibody
    • MGC133098 antibody
    • MGC62412 antibody
    • mKIAA0478 antibody
    • RGD1309866 antibody
    • RP23 95O23.1 antibody
    • ZBT40_HUMAN antibody
    • ZBTB40 antibody
    • Zinc finger and BTB domain containing 40 antibody
    • Zinc finger and BTB domain containing protein 40 antibody
    • Zinc finger and BTB domain-containing protein 40 antibody
    • ZNF923 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ZBTB40 with ab86330 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-ZBTB40 antibody (ab86330) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysates at 50 µg
    Lane 2 : 293T whole cell lysates at 15 µg
    Lane 3 : 293T whole cell lysates at 5 µg

    Developed using the ECL technique

    Predicted band size : 138 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of ZBTB40 by Western Blot of Immunprecipitate.
    ab86330, at 1 µg/ml, staining ZBTB40 in HeLa whole cell lysate immunoprecipitated using ab86330 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 3 minutes.

References

ab86330 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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