Overview

  • Product nameAnti-ZBTB7A antibody
    See all ZBTB7A primary antibodies
  • Description
    Rabbit polyclonal to ZBTB7A
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to a region between residue 525 and the C terminus (residue 584) of Human ZBTB7A (NP_056982.1).

  • Positive control
    • Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Lung Adenocarcinoma, Non Small Cell Lung Cancer, Ovarian Carcinoma, Placenta, Prostate Carcinoma, Stomach Adenocarcinoma.
  • General notesThe concentration of IgG is less than or equal to 1 mg/ml.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab114074 was affinity purified using an epitope specific to ZBTB7A immobilized on a solid support.
  • ClonalityPolyclonal
  • IsotypeIgG

Applications

Our Abpromise guarantee covers the use of ab114074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Antigen retrieval is recommended. Likely to work with frozen sections In some cases, the antibody may be diluted further than indicated.

Target

  • FunctionPlays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3'.
  • Tissue specificityWidely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In tonsil, expressed in squamous epithelium and germinal center lymphocytes (at protein level). Up-regulated in a subset of lymphomas, as well as in a subset of breast, lung, colon, prostate and bladder carcinomas (at protein level).
  • Sequence similaritiesContains 1 BTB (POZ) domain.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp547O146 antibody
    • Factor binding IST protein 1 antibody
    • Factor that binds to inducer of short transcripts protein 1 antibody
    • FBI-1 antibody
    • FBI1 antibody
    • HIV-1 1st-binding protein 1 antibody
    • HIV-1 inducer of short transcripts binding protein antibody
    • HIV-1 inducer of short transcripts-binding factor 1 antibody
    • Leukemia/lymphoma-related factor antibody
    • LRF antibody
    • lymphoma related factor antibody
    • MGC99631 antibody
    • POK erythroid myeloid ontogenic factor antibody
    • Pokemon antibody
    • POZ and Krueppel erythroid myeloid ontogenic factor antibody
    • TIP21 antibody
    • TTF-I-interacting peptide 21 antibody
    • ZBT7A_HUMAN antibody
    • ZBTB7 antibody
    • ZBTB7A antibody
    • Zinc finger and BTB domain containing 7A antibody
    • zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcripts binding protein antibody
    • Zinc finger and BTB domain-containing protein 7A antibody
    • Zinc finger protein 857A antibody
    • Zinc finger- and BTB domain-containing protein 7 antibody
    • ZNF857A antibody
    see all

Anti-ZBTB7A antibody images

  • ab114074 at 1/250 dilution staining ZBTB7A in Formalin-Fixed, Paraffin-Embedded Human Breast carcinoma tissue by Immunohistochemistry. Detection utilised DAB.
  • ab114074 at 1/250 dilution staining ZBTB7A in Formalin-Fixed, Paraffin-Embedded Human Colon carcinoma tissue by Immunohistochemistry. Detection utilised DAB.

References for Anti-ZBTB7A antibody (ab114074)

ab114074 has not yet been referenced specifically in any publications.

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Thank you for contacting Abcam regarding ab114074.


This antibody has not been tested in WB, therefore we do not know if or how well it will work in this application. Generally speaking, I would start by testing several dilutions, rangin...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"