Synthetic peptide within Human ZBTB7A aa 500 to the C-terminus. The exact sequence is proprietary.
Database link: O95365
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab175918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 61 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
For unpurified use at 1/100 - 1/250.
|IP||1/10 - 1/100.|
Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling ZBTB7A with purified ab175918 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Immunofluorescent staining of HeLa cells using labeling ZBTB7A with ab175918 at 1/100 dilution
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ZBTB7A with ab175918 at 1/100 dilution
Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling ZBTB7A with ab175918 at 1/100 dilution
Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using labeling ZBTB7A with ab175918 at 1/10 dilution.