Overview

  • Product name
    Anti-ZBTB7C antibody
  • Description
    Rabbit polyclonal to ZBTB7C
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for ZBTB7C
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
  • Immunogen

    A region within synthetic peptide: MANDIDELIG IPFPNHSSEV LCSLNEQRHD GLLCDVLLVV QEQEYRTHRS, corresponding to N terminal amino acids 1-50 of Human ZBTB7C

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Raji cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

Target

Images

  • Western blot - Anti-ZBTB7C antibody (ab49345)
    Western blot - Anti-ZBTB7C antibody (ab49345)
    Anti-ZBTB7C antibody (ab49345) at 5 µg/ml + Raji cell lysate 10µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 69 kDa
    Observed band size: 59 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Du R  et al. Integrative Mouse and Human Studies Implicate ANGPT1 and ZBTB7C as Susceptibility Genes to Ischemic Injury. Stroke 46:3514-22 (2015). Read more (PubMed: 26542693) »

See 1 Publication for this product

Publishing research using ab49345? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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