Overview

  • Product nameAnti-ZC3H18 antibody
    See all ZC3H18 primary antibodies
  • Description
    Rabbit polyclonal to ZC3H18
  • Tested applicationsSuitable for: IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: SRDRKSGGRL GSPKPERQRG QNSKAPAAPA DRKRQLSPQS KSSSKVTSVP GKASDPGAAS TKSGKASTLS RREELLKQLK AVEDAIARKR, corresponding to amino acids 857-946 of Human ZC3H18.

  • Positive control
    • Human stomach tissue; U 2 OS cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab122673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 106 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.

Target

Anti-ZC3H18 antibody images

  • ab122673, at 1/200 dilution, staining ZC3H18 in paraffin-embedded Human stomach tissue by Immunohistochemistry.
  • ab122673, at 4 µg/ml staining ZC3H18 in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).

References for Anti-ZC3H18 antibody (ab122673)

ab122673 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Feb 03 2014

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Total protein in input 250 µg
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Verified customer

Submitted Feb 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"