Overview

  • Product name
  • Description
    Rabbit polyclonal to ZC3H18
  • Tested applications
    Suitable for: IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: SRDRKSGGRL GSPKPERQRG QNSKAPAAPA DRKRQLSPQS KSSSKVTSVP GKASDPGAAS TKSGKASTLS RREELLKQLK AVEDAIARKR, corresponding to amino acids 857-946 of Human ZC3H18.

  • Positive control
    • Human stomach tissue; U 2 OS cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 106 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.

Target

Anti-ZC3H18 antibody images

  • ab122673, at 1/200 dilution, staining ZC3H18 in paraffin-embedded Human stomach tissue by Immunohistochemistry.
  • ab122673, at 4 µg/ml staining ZC3H18 in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).

References for Anti-ZC3H18 antibody (ab122673)

ab122673 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 03 2014

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Total protein in input
250 µg
Username

Abcam user community

Verified customer

Submitted Feb 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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