Overview

  • Product nameAnti-ZC3H3 antibody
    See all ZC3H3 primary antibodies
  • Description
    Rabbit polyclonal to ZC3H3
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 898-948 of Human ZC3H3, (NP_055932.2)

  • Positive control
    • Whole cell lysate from HeLa and 293T cells.
  • General notesAntibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2 - 5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 101 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similaritiesContains 5 C3H1-type zinc fingers.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA0150 antibody
      • ZC3H3 antibody
      • ZC3H3_HUMAN antibody
      • ZC3HDC3 antibody
      • Zinc finger CCCH domain containing protein 3 antibody
      • Zinc finger CCCH domain-containing protein 3 antibody
      • Zinc finger CCCH type containing 3 antibody
      • Zinc finger CCCH type domain containing 3 antibody
      see all

    Anti-ZC3H3 antibody images

    • All lanes : Anti-ZC3H3 antibody (ab86416) at 0.04 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg
      Lane 2 : HeLa cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg


      Predicted band size : 101 kDa
      Observed band size : 117 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 71 kDa. We are unsure as to the identity of these extra bands.
    • Detection of Human ZC3H3 by Immunoprecipitation on Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86416 at 3 ug/mg lysate. Subsequent WB analysis was performed using ab86416 at 1ug/ml.

    References for Anti-ZC3H3 antibody (ab86416)

    ab86416 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"